重组霍乱毒素B亚基与疟原虫多表位融合蛋白的免疫保护作用研究

Induction of Protective Immune Response in Mice and Rhesus Monkeys by Immunization with Fusion Protein of Cholera Toxin B Subunit and Multiples of Plasmodium falciparum

对以霍乱毒素B亚基为载体蛋白的重组疟疾多价抗原在小鼠及恒河猴中的免疫原性及对相应疟原虫感染的免疫保护作用进行了研究。结果表明 :该抗原免疫小鼠后 ,对约氏疟子孢子攻击的保护率在 5 0 %左右 ;恒河猴免疫后用 1× 10 8食蟹疟裂殖子攻击 ,对照组 2只动物在攻击后 4d感染 ,感染持续 30d以上 ;免疫组 2只动物中 ,两只动物在感染 6～ 7d后完全恢复 ,且 1只推迟 3d感染 。

Recombinant fusion protein of cholera toxin B subunit (CTB) and poly\|valent protective epitopes of plasmodium falciparum was given to i.m to C57BL/6j mice and rhesus monkeys three times. In rhesus monkeys,high level of antibodies for CTB (1:6400) and malaria epitopes (1:3200) amtobpdoes were elicited as well as the specific CTL activity for P. plasmodium . After the mice were challenged with sporozoites of P.yeolli , about 50% of them were protected from the patent infection. A blood\|stage challenge with 10 8 of P.cynomolgi parasite were given to rhesus monkeys, which showed that two animals in control group were patent infection for at least 30 days,in contrast,the two animals immunized were recovered respectively at the day of 11 and 15 after challenges. The results suggested that cholera toxin acts as an effective adjuvent in the development of malaria vaccine.