摘要
PCR method was used to introduce the code sequence of Factor Xa cleavage site to the 5′ end of cecropin CMIV mutant gene X,then the gene was cloned into the expression vector pGEX KG,and was highly expressed in E.coli BL21 by IPTG induction.The fusion protein was purified by affinity chromatography and was cleaved by Factor Xa.Cecropin X with antibacterial activity was obtained after purified by ion exchange chromatography.
PCR method was used to introduce the code sequence of Factor Xa cleavage site to the 5′ end of cecropin CMIV mutant gene X,then the gene was cloned into the expression vector pGEX KG,and was highly expressed in E.coli BL21 by IPTG induction.The fusion protein was purified by affinity chromatography and was cleaved by Factor Xa.Cecropin X with antibacterial activity was obtained after purified by ion exchange chromatography.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第3期411-414,共4页
Chinese Journal of Biotechnology
基金
国家自然科学基金!( 3 95 70 111)
铁道部科学和技术基金!( 96z0 61)资助项目
