CNTF对严重烫伤大鼠海马神经元损伤的保护作用

PROTECTIVE ROLE OF CILIARY NEUROTROPHIC FACTOR ON NEURONAL INJURY IN HIPPOCAMPUS OF BURN RATS

目的 :观察大鼠体表严重烫伤后海马神经元的病理变化 ,探讨CNTF对其保护作用。方法 :SD大鼠侧脑室埋管 ,制成 30 %体表面积Ⅲ度烫伤模型 ;伤后 3d ,测定脑组织含水量 ,海马乳酸脱氢酶 (LDH)和一氧化氮 (NO)的含量 ;做病理切片 ,尼氏染色。结果 :大鼠严重烫伤后 ,脑组织含水量增加 ,海马出现明显的病理变化 ,尼氏小体减少或消失 ,胞体肿胀 ,LDH和NO的含量增加 ;给予CNTF后 ,脑水肿、海马的病理变化有明显改善 ,并能降低LDH和NO的含量。结论 :大鼠严重体表烫伤可造成脑组织损伤 ,CNTF对海马神经元损伤有保护作用 ,这种保护作用可能与其降低NO含量有关。

Aim: The protective role of CNTF on hippocampal neuronal damage induced by scalding was investigated in rats. Methods: The rats bearing cerebro-ventricle intubations, were inflicted with 30% of total body scope area of the animals, full thickness burn injury. The pathological changes, the contents of brain water, the contents of LDH and NO in hippocampus were observed in the rats. Results: Remarkable pathological changes were found in hippocampus: Nissl bodies decreased or disappeared, cyton swelled. The water contents and the level of LDH and NO were increased in scalded rats. CNTF could remarkably improve the pathological changes and reduce the increase of LDH, NO and the water contents. Conclusion: The neurons damage caused by burns may be associa ted with increase of NO. Effect of CNTF on the neuronal injury may be related to decrease of NO.