脐带血清在骨髓造血祖细胞培养中的效应

EFFECTS OF CORD BLOOD SERUM ON COLONY CULTURE OF BONE MARROW HEMATOPOIETIC PROGENITOR CELLS

目的 :探讨人脐带血清 (CBS)在骨髓造血祖细胞培养中的效应。方法 :用人骨髓细胞进行CFU GM、CFU E、BFU E、CFU GEMM培养。结果 :CBS能直接刺激骨髓细胞CFU GM的形成。与血型相同否无关。四人份以上的混合CBS(MCBS) ,刺激活性高且稳定。 1 0 %MCBS相当于 6 5 .6 μg/LGM CSF、0 .2 3、0 .3、0 .46kU/LEpO对CFU GM、CFU E、BFU E、CFU GEMM所起的作用。MCBS可提高CFU GM、CFU E、BFU E、CFU GEMM常规体系的集落产率。在CFU GM培养时 ,2 0 %MCBS与 1 0 0 μg/LGM CSF和 2 0 %马血清具有相同的效果。MCBS可直接刺激CFU E形成。如培养体系内无BpA存在时 ,形成BFU E、CFU GEMM较少或几乎为零 ,当含BpA时 ,可形成一定量的BFU E、CFU GEMM集落。结论 :CBS(MCBS)内 :①含有GM CSF、Epo等物质或因素 ;②具有刺激CFU GM、CFU E、BFU E、CFU GEMM的生长活性 ;③同血型或不同血型 4人份以上混合 ,刺激活性高且稳定。具有良好的应用前景。

Aim: To provide experimental proofs for Cord Blood clinical appliance, the effects of human CBS (Cord Blood Serum) on colony culture of bone marrow hematopoietic progenitor cells were studied. Methods: Observed the colony forming capacity of CFU GM,CFU E,BFU E,CFU GEMM when they were cultivated using hBMNC with CBS, Mixed Cord Blood Serum (MCBS) and/or cytokines. Resutts: 10% CBS could promote the forming of CFU GM of BMC directly, no matter what blood group the CBS or BMC were of. If four or more copies of CBS were mixed (MCBS), no matter whether the “ABO” blood groups between them were same or not, they could produce more CFU GM than single copy or two copies mixed CBS when used, and their activity was stable. MCBS could increase the production of CFU GM、CFU E、BFU E、CFU GEMM in routine culture system, and 10%MCBS corresponds with 65.6 μg/L GM CSF,0.23,0. 3,0.46 kU/L Epo to CFU GM,CFU E,BFU E,CFU GEMM respectively. When used in colony culture of CFU GM,20% MCBS has the same effect as 100 μg/L GM CSF with 20% HS. 20% MCBS could also directly promote the forming of CFU E. It formed few or none BFU E and CFU GEMM without BpA and some BFU E and CFU GEMM when with BpA. Conclusion: ①SCBS (MCBS) contains such substances as GM CSF, EPO, and so on. ②There exists some activities which can promote the forming of CFU GM, CFU E, BFU E, CFU GEMM in CBS (MCBS). ③These activities would be stronger and more stable if four or more copies of any blood group CBS were mixed. It may be a fine prospects of clinical appliance.