摘要
目的和方法 :采用原位杂交技术 ,观察遗传性听源性癫痫易感大鼠海马内CCKmRNA表达的改变及海马CA3区注射CCK 8及其受体阻断剂对大鼠癫痫发作的影响。结果 :①癫痫发作大鼠海马内CCKmRNA表达明显增强(P <0 .0 5 0 .0 1 ) ;但癫痫反复发作的大鼠海马内CCKmRNA表达较癫痫发作一次大鼠明显减少 (P <0 .0 5 0 .0 1 ) ;②海马CA3 区注射CCK 8或L 36 4+CCK 8,大鼠癫痫发作明显减轻 (P <0 .0 5 ) ;海马CA3 区注射L 36 5后 ,CCK 8压抑癫痫发作的作用消失 (P <0 .0 1 )。结论 :CCK 8参与癫痫发作过程 ,且具有压抑癫痫发作作用 ,该作用可能是通过CCK B受体而实现的。
Aim and Methods:The effect of CCK 8 and its receptor blocker on seizure and CCK mRNA expression in hippocampus were studied by means of in situ hybridization and microinjection in genetically epilepsyprone rats. Results:① CCK mRNA expression was significantly increased in hippocampus after single seizure ( P <0.05 0.01); CCK mRNA expression was significantly decreased after multiple consecutive seizure compared with a single seizlre group ( P <0.05 0.01).② After injecting CCK 8 or L 364 + CCK 8 into hippocampus CA 3 area, seizure was significantly inhibited( P <0.05).But after injecting L 365+CCK 8 into hippocampus CA 3 area,the effect of CCK 8 on seizure was disappeared ( P >0.01). Conclusion: CCK 8 was involved in epilepsy seizure, CCK 8 can inhibit epilepsy seizure and its effect may be have much to do with CCK B receptor.
出处
《中国应用生理学杂志》
CAS
CSCD
2000年第1期75-78,共4页
Chinese Journal of Applied Physiology
基金
山东省教育委员会基金!(J95K3 4 )
