维拉帕米对大鼠浸水应激性胃溃疡的影响

EFFECT OF VERAPAMIL ON GASTRIC ULCER INDUCED BY RESTRAINT WATER-IMMERSION IN RATS

目的和方法 :利用浸水应激性胃溃疡模型 ,研究维拉帕米ip对大鼠应激性胃溃疡的影响及其作用机制。 结果 :①维拉帕米 (5～ 2 0mg/kg)可抑制大鼠浸水应激性胃溃疡的发生 ;②维拉帕米 (1 0mg/kg)可抑制浸水应激大鼠胃液 ,胃酸的分泌及胃的运动 ;而对胃粘液的分泌无影响 ;③浸水应激后大鼠胃粘膜一氧化氮合成酶 (NOS)活性和一氧化氮 (NO)含量均明显降低 ,维拉帕米 (1 0mg/kg)可抑制应激导致的NOS活性和NO含量的降低。结论 :维拉帕米有抗大鼠浸水应激性胃溃疡的作用 ,其机制与抑制胃酸分泌 ,胃的运动 ,胃粘膜NOS活性和NO含量的降低有关 ,而与胃粘液的分泌无关。

Aim and Methods: In the restraint water immersion rats, we studied the protection of verapamil administered intraperitoneally on gastric ulcer and its mechanism. Results: ①Verapamil (5～20 mg/kg) significantly inhibited the formation of gastric ulcer induced by restraint water immersion. ② Verapamil (10 mg/kg) suppressed the gastric juice secretion, gastric acid secretion and gastric motility in restraint water immersion rats, but had no effect on gastric mucosa secretion. ③ In restraint water immersion rats, gastric mucosal NOS activity and NO content were decreased significantly and verapamil (10 mg/kg) could inhibit the decrease of NOS activity and NO content. Conclusion: Verapamil could inhibit gastric ulcer induced by restraint water immersion and its protection might be related to inhibit gastric acid, motility, decrease of NOS activity and NO content, but was not related with gastric mucus.