摘要
比较了产朊假丝酵母细胞与分离纯化的细胞壁对铜离子吸附能力。观察铜离子浓度、温度和pH值对产朊假丝酵母吸附铜离子的影响，探讨细胞壁在酵母吸附重金属离子过程中的作用机理。结果表明，细胞壁是酵母吸附重金属离子的主要部位。细胞壁的蛋白酶酶解实验证明，对胰蛋白酶不敏感的细胞壁嵌合蛋白是铜离子吸附的主要位点。
The ion adsorption capacity differences of cell wall of Candida utilis and isolated purified cell wall of the yeast was studied by comparison. The effect of ion concentration, temperature and pH to the ion adsorption of Candida utilis were observed, and affect mechanism of the process was discussed. The results showed that cell wall of yeast were the main part for heavy metal ion accumulation. Treatments of cell wall with proteinase demonstrated that main sites for heavy metal ion accumulation are the trypsin- insensitive integral proteins.
出处
《微生物学杂志》
CAS
CSCD
2000年第1期11-14,共4页
Journal of Microbiology
基金
国家自然科学基金!39500074
关键词
产朊假丝酵母
细胞壁
嵌合蛋白
铜离子
吸理机理
Candida utilis, cell wall, cell wall integral proteins, copperion adsorption mechanism
