银杏叶提取物对慢性阻塞性肺疾病大鼠炎症反应的影响

Effect of Ginkgo Biloba Extract on Inflammatory Reaction in Rats With Chronic Obstructive Pulmonary Disease

[目的]探讨银杏叶提取物(GBE)对慢性阻塞性肺疾病(COPD)大鼠血清、肺泡灌洗液(BALF)中C反应蛋白(CRP)的含量及气道炎症的影响。[方法]Wistar大鼠40只,随机分为正常对照组(A组)、模型组(B组)、GBE早期干预组(C组)、GBE后期干预组(D组)。B、C和D三组采用烟熏加脂多糖(LPS)及冷空气刺激复合因素造模法建立COPD大鼠模型,C和D组分别在1～14 d、29～42 d腹腔注射银杏叶提取物注射液,43 d后对各组大鼠肺组织行病理学、血清及BALF中CRP含量的检测。[结果]①B、C、D三组均有COPD特征性改变,但程度不同。②气道炎症病变:与A组比较,B组气道各炎症病理积分显著增高(P<0.05或P<0.01);与B组比较,C、D组病理积分明显减少(P<0.05或P<0.01),而D组积分高于C组(P<0.05)。③血清及肺泡灌洗液CRP含量:与A组比较,B组CRP含量显著升高(P<0.01);与B组比较,C组CRP含量明显著降低(P<0.01),而D组CRP含量亦有降低(P<0.05)。[结论]GBE可以降低血清及BALF中CRP的表达,可抑制气道炎症反应,提示GBE具有抑制COPD气道及全身炎症反应的作用。

Obstract： [Objective] To investigate the effect of ginkgo bilogo extract （GBE） on airway inflammation in rats with chronic ob- structive pulmonary disease （COPD）, as well as C-reactive protein （CRP） in serum and bronchoalveolar lavage fluid （BALF）. [Methods] Forty Wistar rats were randomly divided into the following four groups, normal control group （group A）, model group （group B）, GBE early intervention group （group C）, and GBE late intervention group （group D）. In group B, C and D, COPD model was established through comprehensive factors of smoking, lipopolysaccharide using, and cold air stimulation. In group C and D, in- traperitoneal injection of GBE was given during 1-14 d and 29~42 d in the treatment, respectively. 43 days later, pathological changes in lung tissues, as well as CRP in serum and BALF were detected in all groups. [Results] （~）Specific changes of different degree of COPD occurred in group B, C and D. ~）Compared with group A, pathological scores of airway inflammation in group B sig- nificantly increased （P 〈0.05 or P 〈0.01 ）. Compared with group B, pathological scores of airway inflammation in group C and D greatly reduced （P 〈0.05 or P 〈0.01 ）, and those in group D were higher than those in group C （P 〈0.05）. ~）Compared with group A, CRP in serum and BALF in group B greatly increased （P 〈0.01 ）. Compared with group B, CRP in serum and BALF in group C significantly decreased （P 〈0.01 ）, and reduced too in group D （P 〈0.05）. [ Conclusion ] GBE can lower C RP expression in serum and BALF, and inhibit airway inflammatory reaction, indicating that GBE can control airway inflammation of COPD and systemic in- flammatory reaction.