HPLC法同时测定青叶胆中獐牙菜苦苷、龙胆苦苷和獐牙菜苷的含量

HPLC Simultaneous Determination of Swertiamarin Gentiopicroside and Sweroside in Swertia mileensis

目的:建立青叶胆中獐牙菜苦苷、龙胆苦苷、獐牙菜苷的含量测定方法,为控制其质量提供科学依据。方法:采用高效液相色谱法。色谱条件:色谱柱为Phenomsil C18色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.02%磷酸溶液(23∶77);检测波长为243 nm;流速为1 mL/min;柱温为30℃。结果:3种成分均达到基线分离,獐牙菜苦苷、龙胆苦苷、獐牙菜苷的线性范围分别为0.33～1.98μg(r=0.9997),0.16～0.96μg(r=0.9999),0.20～1.20μg(r=0.9997),回收率分别为100.2%,99.5%,98.2%。结论:该法快速、准确、稳定,可用于青叶胆药材质量评价。

Objective： To determinate the contents of the iridoids, swertiamarin, gentiopicroside and sweroside in Swertia mileensis. Methods：HPLC was used. The samples were separated on a phenomsil C18 column （250 mm×4.6 mm, 5 μm）with which eluted with methanol and water（0.02% phosphoric acid）. The ratio of methanol and water was 23：77. The gradient elution with flow rate was set at 1.0 mL/min, with UV detection at 243 nm and column temperature at 30 ℃. Results ： Thee eoilpounds were i- solated on base line. The linear ranges of swertiamarin, gentiopicroside and sweroside were 0.33 - 1.98 μg（ r = 0. 9997 ） ,0. 16 0. 96μg （ r = 0.9997） ,0. 20 - 1.20 μg （ r = 0. 9997 ）, respectively. The average recoveries were 100. 2 % .99.5 % and 98.2 %. respectively. Conclusion：The method has good reproducibility, and can be used in setting up the method to determinate the contents and quality control of Swertia mileensis from various habitats.