灯盏花素对肾损害模型小鼠肾脏TGF-β_1、Col-Ⅳ表达的影响

Effect of Breviscapine on the Expressions of TGF-β_1 and Col-Ⅳ in Mice with Renal Impairment

目的:研究灯盏花素对肾损害模型小鼠肾功能和转化生长因子(TGF)-β1、Ⅳ型胶原蛋白(Col-Ⅳ)表达的影响。方法:腹腔注射顺铂8mg/kg以复制小鼠肾损害模型。实验分为对照(5%羧甲基纤维素钠)、模型(5%羧甲基纤维素钠)与灯盏花素高、低剂量(50、25mg/kg)组。灌胃给药,每天1次,连续7d。给药结束后HE染色观察大鼠肾组织改变,进行肾脏指数、尿蛋白(Upr)/尿肌酐(Ucr)比值、N-乙酰氨基葡萄糖苷酶(NAG-U)等肾功能指标测定;免疫组化法检测肾组织TGF-β1、Col-Ⅳ的表达。结果:病理切片显示高、低剂量灯盏花素可显著改善顺铂致肾损害小鼠的肾组织结构。与模型组比较,灯盏花素高、低剂量组小鼠肾脏指数、Upr/Ucr比值与NAG-U活性均显著下降(P<0.01或P<0.05),Col-Ⅳ表达显著增强(P<0.05);灯盏花素高剂量组小鼠肾组织TGF-β1表达显著下降(P<0.05)。结论:灯盏花素对顺铂致肾损害小鼠肾脏具有保护作用,其机制可能与下调肾组织TGF-β1及上调Col-Ⅳ的表达有关。

OBJECTIVE：To study the effect of breviscapine on renal function and the expression of TGF-β1 and Col-Ⅳin mice with renal impairment.METHODS：Renal impairment model was induced by intraperitoneal injection of cisplatin（8 mg/kg）.Mice were randomly divided into control group（5% CMC-Na）,model group（5% CMC-Na）,breviscapine high-dose and low-dose groups（50,25 mg/kg）.They were given relevant medicine intragastrically once a day for consecutive 7 days.Changes of renal tissue was observed by HE staining after medication,and renal index,ratio of Upr to Ucr,NAG-U and other renal function index were determined;the expression of TGF-β1 and Col-Ⅳ in renal tissues were determined by immunohistochemical method.RESULTS：Pathological examinations showed that high-dose,low-dose breviscapine improved the renal tissue of mice with cisplatin-induced renal impairment.Compared with model group,renal index,Upr/Ucr ratio and NAG-U were significantly down-regulated in breviscapine high-dose and low-dose groups（P〈0.01 or P〈0.05）,and the expressions of Col-Ⅳincreased（P〈0.05）.The expressions of TGF-β1 in breviscapine high-dose group was significantly decreased（P〈0.05）.CONCLUSION：Breviscapine could improve the kidney functions of mice with renal impairment,which may be related to the down-regulation of the expressions of TGF-β1 and up-regulation of the expression of Col-Ⅳ.