高速逆流色谱法分离丁公藤中东莨菪内酯

Isolation of Scopoletin from Erycibes schmidtii by High-speed Counter-current Chromatography

目的:探讨以高速逆流色谱(HSCCC)法分离丁公藤中东莨菪内酯的方法。方法:用HSCCC法对丁公藤粗提物进行分离,分离条件:两相溶剂系统为正己烷-乙酸乙酯-甲醇-水(3:5:3.5:5,V/V/V/V),上相为固定相,下相为流动相,转速为850r/min,流速为2ml/min,水浴温度为25℃。结果:通过红外光谱、质谱和核磁共振分析,得到的产物是东莨菪内酯;经高效液相色谱测定,产物纯度为97.89%。结论:该法可对丁公藤中东莨菪内酯进行快速有效地分离。

OBJECTIVE：To explore the isolation of scopoletin from Erycibes schmidtii by high-speed counter-current chromatography（HSCCC）.METHODS：The crude extract of E.schmidtii was isolated by HSCCC,and the isolation conditions were as followd：the two phase solvent system was composed of n-hexane-ethyl acetate-methanol-water（3：5：3.5：5,V/V/V/V）,the upper phase was used as the stationary phase,and the lower phase as the mobile phase,the apparatus rotated at 850 r/min,the flow rate was 2 ml/min,while the bath temperature was 25 ℃.RESULTS：The product isolated by HSCCC was scopoletin identified by IR,MS and NMR,and the purity of product was 97.89% by HPLC.CONCLUSION：HSCCC can be used to isolate scopoletin from E.schmidtii efficiently and quickly.