RP-HPLC法同时测定补阳还五汤中3种指标成分的含量

Simultaneous Determination of 3 Index Components in Buyang Huanwu Decoction by RP-HPLC

目的:建立同时测定补阳还五汤中芍药苷、毛蕊异黄酮苷、阿魏酸含量的方法。方法:采用反相高效液相色谱法。色谱柱为OdyssilC18(250mm×4.6mm,5μm),流动相为乙腈-水-0.1%磷酸(16:64:20,V/V/V),检测波长为230nm(芍药苷)、260nm(毛蕊异黄酮苷)、328nm(阿魏酸),流速为1.0ml/min,柱温为30℃。结果:芍药苷、毛蕊异黄酮苷、阿魏酸的进样量分别在0.0964～0.9640μg(r=0.9992)、0.0990～0.9900μg(r=0.9996)、0.00836～0.0836μg(r=0.9994)范围内与各自峰面积积分值呈良好线性关系;三者的平均加样回收率分别为99.72%、99.14%和97.96%,RSD分别为0.5%、1.1%和1.4%(n均为6)。结论:本方法简便、快速、灵敏度高、重复性好,适用于补阳还五汤的质量控制。

OBJECTIVE：To establish the method for simultaneous determination of paeoniflorin,calycosin and ferulic acid in Buyang huanwu decoction.METHODS：RP-HPLC method was adopted.The determination was performed on Odyssil C 18（250 mm× 4.6 mm,5 μm）column with mobile phase consisted of acetonitrile-water-0.1% phosphate acid（16：64：20,V/V/V）at the flow rate of 1.0 ml/min.The detection wavelength was set at 230 nm（paeoniflorin）,260 nm（calycosin）and 328 nm（ferulic acid）.The column temperature was 30 ℃.RESULTS：The linear ranges were 0.096 4-0.964 0 μg（r=0.999 2）for paeoniflorin,0.099 0-0.990 0 μg for calycosin（r=0.999 6）and 0.008 36-0.083 6 μg for ferulic acid（r=0.999 4）,respectively.The average recoveries were 99.72% for paeoniflorin（RSD=0.5%,n=6）,99.14% for campanulin（RSD=1.1%,n=6）and 97.96% for ferulic acid（RSD=1.4%,n= 6）.CONCLUSION：The method is simple,quick,sensitive and reproducible for the quality control of Buyang huanwu decoction.