贵州两产地石楠藤中挥发油的GC-MS分析

Analysis of Volatile Oil of Piper wallichii from 2 Producing Areas of Guizhou Province by GC-MS

目的:分析贵州贵阳相思河与赤水桫椤所产石楠藤中挥发油成分并进行比较。方法:采用水蒸气蒸馏法提取石楠藤中挥发油,气相色谱-质谱(GC-MS)联用技术测定挥发油成分,面积归一化法计算各成分的相对质量分数。结果:从贵阳相思河产石楠藤中共分离出61种成分,已鉴定54种,其相对质量分数占挥发油总量的77.48%;质量分数较高的成分有双环大牻牛儿烯(8.846%)、α-律草烯(6.230%)、δ-荜澄茄烯(5.410%)。从赤水桫椤石楠藤中共分离出58种成分,已全部鉴定;质量分数较高的成分有β-细辛醚(24.352%)、双环大牻牛儿烯(9.979%)、β-石竹烯(7.630%)。两者共有成分45种,均含有较多的萜类、醇类,但其相对质量分数有较大差异。结论:石楠藤中挥发油成分的差异与其产地、生长环境密切相关。本试验结果可为进一步开发利用石楠藤资源提供理论依据。

OBJECTIVE：To analyze and compare the volatile oil of Piper wallichii from Guiyang Xiangsihe and Chishui Suoluo.METHODS：The volatile oil was extracted from P.wallichii by steam distillation and the chemical components of them were identified by GC-MS and relative content of the components were calculated with normalization method.RESULTS：61 compounds were extracted from P.wallichii in Xiangsihe of Guiyang,among which 54 compounds were identified,and the relative content accounted for 77.48% of the total volatile oil;the high content of components were bicyclogermacrene（8.846%）,α-humulene（6.230%）and δ-cadinene（5.410%）.58 compounds were extracted and had identified from P.wallichii in Chishui Suoluo.The high content of components were β-Asarone（24.352%）,bicyclogermacrene（9.979%） and β-caryophyllene（7.630%）.45 compounds were common in both,and usually contained plenty of terpenoid and alcohols,but there was great difference of relative content.CONCLUSION：The difference of volatile oil from P.wallichii is closely correlated to producing area and growing environment.The results of the studies can provide reference for further study and development of P.wallichii resources.