摘要
用甲基磺酸乙酯诱变处理常规粳稻浙粳88,获得了矮秆水稻突变体Zj88d,多代自交稳定遗传。遗传分析表明突变体Zj88d的矮秆性状受一对隐性核基因控制。采用图位克隆方法,将目的基因定位在水稻第7染色体短臂末端7-44w和RM5344间的603kb区间。排查该区间包含的100个候选基因,发现已克隆的矮秆基因OSH15也位于其中。测序结果显示,突变体Zj88d中的OSH15基因在第2内含子最末位发生"g→a"单碱基突变。
The stably inherited dwarf mutant Zj88d was produced by treating the dry seeds of japonica rice variety Zhejing 88 with ethyl methylsulfonate（EMS）.Genetic analysis of the dwarf mutant Zj88d revealed that this trait is controlled by one pair of recessive nuclear gene.The gene was located in the interval of 603 kb between 7-44w and RM5344 markers on the short arm of chromosome 7 by map-based cloning.The dwarf gene ＂OSH15＂ was identified by screening one hundred candidate genes on the target chromosomal segment.Sequencing results further revealed that Zj88d gene has a single-base substitution（g to a） in the last nucleotide of intron 2 of OSH15 allele.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2013年第1期35-40,共6页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31071207
31100142)
浙江省科技项目(2007C12902
2010R50033)
公益性行业(农业)科研专项(201103007)
关键词
水稻
矮秆
等位基因
单碱基突变
选择性剪切
rice
dwarf
allele
single-base substitution
alternative splicing
