和厚朴酚调控P38信号通路诱导人宫颈癌Hela细胞凋亡的实验研究

Honokiol Mediates the Apoptosis of Human Cervical Carcinoma Hela Cells by Regulating P38 Signal Pathway

目的探讨和厚朴酚诱导人宫颈癌Hela细胞的凋亡及激活P38信号通路之间的关系。方法采用细胞培养技术,用一定浓度的药物处理细胞。应用蛋白印迹技术检测聚腺苷二磷酸核糖聚合酶(PARP)及其切割蛋白水平,聚腺苷二磷酸核糖聚合酶切割蛋白的出现代表细胞起始凋亡;应用蛋白印迹技术检测P38蛋白的磷酸化水平,P38蛋白的磷酸化水平的上调表示P38信号通路的激活。利用抑制剂SB203580阻断P38信号通路,通过这个方法检测和厚朴酚诱导细胞凋亡的变化情况。结果和厚朴酚激活P38信号通路与诱导细胞凋亡之间有一定联系。结论和厚朴酚通过调控P38信号通路诱导Hela细胞的凋亡。

Objective To research the relationship between honokilo inducing the apoptosis of human cervical carcinoma Hela cells and activating P38 signal pathway.Methods The cells,which were cultured by cell culture technique,were treated with the drug.The protein level of the poly ADP-ribose polymerase protein and its cleaved fragment were detected by Western-Blotting assays.The appearance of its cleaved fragment indicated the starting of the apoptosis.The phosphorylation of the protein P38 was detected by Western-Blotting assays,the level of its phosphorylation indicates the activation of P38 signal pathway.The P38 signal pathway was blocked by the inhibitor SB203580,the change of honokiol inducing cell apoptosisi was detected with the approach.Results Honokiol activated P38 signal pathway and induced cell apoptosis.Conclusion Honokiol induces the apoptosis of Hela cells by regulating P38 signal pathway.