手针针刺时程对大鼠痛阈影响的实验观察

The Experimental Observation of Acupuncture Time Taking on the Effects of Pain Threshold in Rats

目的通过手针针刺不同时程对大鼠痛阈影响的实验观察,了解合适的针刺时程在建立针刺动物实验模型以及临床针刺治疗中的科学作用。方法分别采用平补平泻手法针刺SD大鼠"后三里"穴5,10,15,20,25 min,采用热水甩尾测痛方法检测大鼠针刺前后的痛阈,利用统计学STATA 10.0处理分析数据。结果①针刺5 min,针刺前痛阈值(6.12±0.26)针刺后痛阈值(7.95±0.27);针刺10 min,针刺前痛阈值(5.31±0.51)针刺后痛阈值(9.27±0.44);针刺15 min,针刺前痛阈值(5.44±0.24)针刺后痛阈值(10.57±0.45);针刺20 min,针刺前痛阈值(5.29±0.22)针刺后痛阈值(10.34±0.34);针刺25 min,针刺前痛阈值(4.55±0.32)针刺后痛阈值(9.87±1.01);经配对t检验比较,均显示出有意义的差异(p<0.01)。②针刺5 min后痛阈值(7.95±0.27)与针刺10 min后痛阈值(9.27±0.44)配对t检验比较,有统计学意义的改变(P<0.05);针刺5 min后痛阈值(7.95±0.27)与针刺15 min后痛阈值(10.57±0.45)配对t检验比较,具有显著意义的改变(P<0.05)。③针刺15min后痛阈值(10.57±0.45)与针刺20 min后痛阈值(10.34±0.34)以及针刺25min后痛阈值(10.8±0.89)分别配对t检验比较,未见有统计学意义的改变(P=0.658 3;P=0.686 2)。结论手针平补平泻针刺大鼠"后三里"腧穴15 min、即可达到较佳的调节大鼠痛阈的针刺效果。

Objective Through the experimental observation of acupuncture with different duration time to the effects of on pain threshold in rats and know the scientific foundation for further study on the establishment of animal experimental model and the Clinical Acupuncture Acupuncture Treatment of Science in action.Methods Use acupuncture mild reinforcing-reducing method on Housanli Point（ST36） in rats（5,10,15,20,25 min）,various acupuncture point prescriptions were used to gather different data of pain-threshold with the methods of investigating tail-flick latency,and the data were analyzed statistically.Results（1）Under paired t-test：the pain threshold 5 min before acupuncture（6.12±0.26） was significantly（P0.01） different from that after acupuncture（7.95±0.27）;the pain threshold 10 min before acupuncture（5.31±0.51） was significantly（p0.01） different from that after acupuncture（9.27±0.44）;the pain threshold 15 min before acupuncture（5.44±0.24） was significantly（P0.01） different from that after acupuncture（10.57±0.45）;the pain threshold 20 min before acupuncture（5.29±0.22） was significantly（P0.01） different from that after acupuncture（10.34±0.34）;the pain threshold 25 min before acupuncture（4.55±0.32） was significantly（P0.01） different from that after acupuncture（9.87±1.01）;（2） Under paired t-test：the pain threshold 5 min after acupuncture was significantly（P0.05） different from that 10 min after acupuncture;the pain threshold 5 min after acupuncture was significantly（P0.05） different from that 15 min after acupuncture;（3） Under paired t-test：there was no statistical difference（P=0.6583;P=0.6862） between the pain threshold 15 min after acupuncture,20 min after acupuncture and 25 min after acupuncture.Conclusion Acupuncture with mild reinforcing-reducing method on Housanli Point（ST36）15 min,can achieve better regulation of acupuncture effect on pain threshold in rats.