山羊磷脂氢谷胱甘肽过氧化物酶生物信息学分析

Bioinformatis Analysis of PHGPx Gene in Goat(Capra hircus )

旨在克隆山羊磷脂氢谷胱甘肽过氧化物酶(PHGPx)基因cDNA全序列并进行序列分析。提取山羊睾丸中总RNA,利用RT-PCR和RACE技术,扩增山羊PHGPx基因cDNA序列并对其生物信息学进行分析。结果表明,山羊PHGPx基因cDNA序列全长844 bp,共编码199个氨基酸;山羊与牛、猪、人和小鼠的氨基酸序列同源性均大于90%;山羊PHGPx蛋白二级结构功能区域属谷胱甘肽过氧化物酶家族,预测23-24和28-29氨基酸位点有潜在的信号肽位点;UPGMA算法构建该物种间分子系统进化树,山羊与牛先聚为一类,再分别与猪、鼠、人、鸡聚类,最后与蜜蜂聚类,与物种动物学分类基本吻合。首次克隆了山羊PHGPx基因,具有GSH-Px家族典型特征,研究结果将为PHGPx基因表达分子调控研究提供一定的理论依据。

This study aimed to clone the full length cDNA of goat PHGPx gene. Total RNA was extracted from goat testis and the full length cDNA was obtained by the reverse transcription PCR and RACE method. The results demonstrated that the full length of goat PHGPx cDNA was 844 bp, which encode 199 amino acids. The multiple sequence alignments revealed that the identity of goat PHGPx amino acids sequence were above 90% with other species. Bioinformatics analysis indicated that goat PHGPx belonged to GSH-Px family in the secondary structure,＂ and predicted the signal peptide at the position of 23-24 or 28-29. The molecular phylogenetic trees among species according the UPGMA method indicated that goat and cow assembled, and then assembled with pig, rat, human and chicken, respectively and assembled with bee last. This result of phylogenetic clustering was identical to the zoological classification. In summary, Capra hircus PHGPx gene has been cloned, and it＇s concluded that PHGPx has typical characteristics with the homologous genes in GSH-Px family. These results would be the theoretical foundation for the further study on the genetic control of PHGPx gene expression.