一株油藏耐热原油降解菌的分离鉴定与特性分析

Isolation,Identification and Characterization of a Crude-oil-degradating Thermophiles in Petroleum Reservoirs

从中原油田高温采出液中分离到一株能在55℃高温条件下利用石油烃类生长的耐高温菌株P98-18A1。基于表型、生理生化特性和16S rDNA序列分析,初步鉴定该菌株为芽孢杆菌属(Bacillus sp.)。通过菌株对原油及部分烷烃降解试验得出,该菌株在适宜条件下,能有效地利用C_6-C_(16)的正构烷烃生长,对链长大于C_(16)的烷烃在某种程度上具有一定的选择性利用能力。

A high temperature-resistant bacteria strain P98-18A1 capable of growing in petroleum hydrocarbons at 55℃ was isolated from hot produced fluid from Zhongyuan oilfield. This strain was identified as Bacillus sp. based on phenotypic, physiological, biochemical and 16S rDNA sequence analysis. With crude oil and several alkanes degradation experiments, results showed that this strain can effectively utilize C6- C16 n-alkanes, and selectively utilize of the chain longer than C16 alkanes to some extent under the optimum conditions.