湖北省黄州区三带喙库蚊抗药性调查及武昌罗索线虫感染对其影响研究

Resistance of Culex tritaeniorhynchus in Huangzhou district,Huanggang city,Hubei province,China to commonly used insecticides and the potential effect of Romanomermis wuchangensis infection on its insecticide resistance

目的了解湖北省黄冈市黄州区近郊三带喙库蚊对常用化学杀虫剂的抗性现状,研究武昌罗索线虫的寄生对其抗性的影响,为蚊虫治理和疾病预防提供技术支撑。方法采用生物测定法测定湖北省黄州区一中(QYZ)感染了武昌罗索线虫的三带喙库蚊种群和东方广场(DFGC)未感染的三带喙库蚊种群对常用杀虫剂半数致死浓度(LC50),以判定抗性等级。结果 DFGC种群对对硫磷、毒死蜱、敌敌畏、残杀威、巴沙、氯菊酯、高效氯氰菊酯、溴氰菊酯和胺菊酯9种杀虫剂的LC50分别为1.85474、0.29574、24.86510、2.17981、6.13724、0.04087、0.01669、0.00960和2.07132mg/L;DFGC种群的LC50均高于QYZ种群,比率为1～7倍。与敏感品系相比,黄州区三带喙库蚊对敌敌畏、残杀威、氯菊酯、高效氯氰菊酯和溴氰菊酯的抗性比率分别为388、9、11、8和24倍。结论黄州区近郊三带喙库蚊对常用化学杀虫剂抗性较高,而武昌罗索线虫的寄生可能是显著降低其对杀虫剂抵抗性的原因。

Objective To investigate the resistance of Culex tritaeniorhynchus in the suburbs of Huangzhou district,Huanggang city,Hubei province,China to commonly used insecticides and the effect of Romanomermis wuchangensis infection on its insecticide resistance,and to provide technical support for mosquito control and disease prevention.Methods The LC50 values of commonly used insecticides for Cx.tritaeniorhynchus infected with R.wuchangensis from the First Middle School of Huangzhou（QYZ） and the uninfected Cx.tritaeniorhynchus from the Dongfang Square（DFGC） were measured by bioassay to evaluate their insecticide resistance levels.Results The LC50 values of parathion,chlorpyrifos,dichlorvos,propoxur,fenobucarb,permethrin,beta-cypermethrin,deltamethrin,and tetramethrin for the uninfected Cx.tritaeniorhynchus in DFGC were 1.85474,0.29574,24.86510,2.17981,6.13724,0.04087,0.01669,0.00960,and 2.07132 mg/L,respectively,1-7 times that of the infected Cx.tritaeniorhynchus in QYZ.Compared with susceptible strains,the Cx.tritaeniorhynchus in Huangzhou had resistance ratios of 388,9,11,8,and 24 for dichlorvos,propoxur,permethrin,beta-cypermethrin,and deltamethrin,respectively.Conclusion The Cx.tritaeniorhynchus in the suburbs of Huangzhou district,Huanggang city,Hubei province,China has relatively high resistance to commonly used insecticides,and the R.wuchangensis infection may contribute to its significant insecticide resistance reduction.