早中晚熟结球甘蓝不同部位组织的再生特性

Study of Regeneration Characteristics about Different Tissues of Different Mature Type Cabbages

对3种不同熟性甘蓝进行培养,以探讨品种不同熟性、不同外植体、不同培养基激素水平等因素对结球甘蓝不定芽诱导、不定芽增殖的影响。结果表明,3种培养基中MS培养基最适于甘蓝组织培养,在MS培养基上京丰甘蓝的下胚轴在激素水平为NAA 0.45mg.L-1和6-BA 3.0mg.L-1时的不定芽分化率最高(76.7%),中甘十一的下胚轴和晚丰甘蓝带柄子叶在NAA 0.6mg.L-1和6-BA 4.5mg.L-1时不定芽分化率最高,分别为83.3%和90%;京丰、晚丰和中甘十一的不定芽在激素分别为NAA 0.3mg.L-1和6-BA7.5mg.L-1、NAA 0.6mg.L-1和6-BA 7.5mg.L-1、NAA 0.3mg.L-1和6-BA 1.5mg.L-1时增殖系数分别达到3.69、4.125和3.45。可见,不同熟性甘蓝的不同部位组织不定芽的产生对基础培养基和激素配比具有比较严格的选择性。

The factors of maturity,explants,hormone combination and mass concentration and medium were investigated on tissue rapid reproduction technology of cabbage in different mature types.The results indicated that the MS medium was fit for tissue culture of cabbage.The rate of adventitious shoots occurrence reached 76.7% induced from hypocotyls of Jingfeng in MS＋NAA 0.45 mg·L-1,6-BA 3.0 mg·L-1.The rate of adventitious shoots occurrence reached 83.3% and 90% induced from hypocotyls of Zhongganshiyi and cotyledon petiole of Wanfeng in MS＋NAA 0.45 mg·L-1,6-BA 3.0 mg·L-1.The adventitious shoots multiplication coefficient of Jingfeng,Wanfeng,Zhongganshiyi was 3.69,4.125,3.45 on MS supplement with NAA 0.3 mg·L-1,6-BA 7.5 mg·L-1;NAA 0.6 mg·L-1,6-BA 7.5 mg·L-1 and NAA 0.3 mg·L-1,6-BA 1.5 mg·L-1,respectively.It was found that more stringent selection to different basic medium and hormone combinations was existed in adventitious shoots occurrence of different tissue in different mature type cabbages.