牛Meg9基因序列及结构的预测与分析

Prediction and analysis of the cattle Meg9 gene sequence and structure

应用生物信息学方法对牛Meg9基因的序列进行预测及分析,发现牛Meg9基因共含有3个外显子,存在3种可变剪切体。与鼠、人和羊序列进行相似性分析,发现牛Meg9基因与绵羊相似性最高,为92%。应用在线软件对牛Meg9基因启动子区及转录因子结合位点进行预测,发现启动子可能位于5′侧翼区1 903～1 953bp处,有16种转录因子结合位点。CpG Island在线软件分析发现牛Meg9基因5′侧翼区和内含子1处共有3个CpG岛。以上研究结果将为研究牛Meg9基因的功能及印记的分子机理奠定基础。

The cattle Meg9 gene is composed of 3 exons and 3 alternative splicings predicted with NCBI,UCSC databases.Sequence homology analysis shows that cattle Meg9 gene shares high identity to sheep（92%）.The potential promoter of cattle Meg9 gene is predicted to locate in 1 903~1 953 bp of 5′-flanking region and contained 16 kinds of potential transcription factor binding sites.Three CpG islands are found in 5′-flanking region and intron 1 of cattle Meg9 gene.These results are essential to analyze the biological function and the molecular regulation mechanism of cattle Meg9 gene.