食品动物源沙门菌的分离鉴定及耐药性检测

Isolation,identification and antimicrobial susceptibility testing of Salmonella isolates from different food-animals

为了解食品动物源沙门菌血清型分布及耐药状况。采用生化鉴定、血清学及沙门菌invA基因PCR方法对分离自不同食品动物源菌株进行沙门菌鉴定;采用微量肉汤稀释法进行菌株对20种药物的敏感性试验。结果显示,本次研究共鉴定出316株沙门菌,14个血清型,以鸡白痢、菲利斯河沙门菌和吉韦沙门菌为主;316株菌株对20种药物呈不同水平的耐药性,对氨苄西林、阿莫西林/克拉维酸、链霉素、四环素、多西环素、奈啶酸、磺胺异噁唑等药物耐药率超过40%;95.5%的菌株呈多重耐药性;猪源、牛源菌株耐药性较禽源严重。以上情况说明,本次分离的食品动物源沙门菌血清型分布广,菌株耐药性较为严重。临床上应把生长周期长的动物体内细菌作为耐药性检测重点,在食品动物生产上应谨慎使用抗菌药物。

To investigate the serotype distribution and resistance in Salmonella isolates from different food-animals, methods including biochemical test, serological test and PCR for invA gene were used to identify Salmonella isolates; Susceptibility testing of isolates to 20 antimicrobials were performed using the broth microdilution methods. The results showed that 316 Salmonella isolates and 14 serotypes were iden- tified, and the main serotypes of strains were S. Pullorum, S. Fyris and S. Give~ 316 isolates showed dif- ferent resistant levels to 20 antimicrobials, more than 40~ isolates were resistant to ampicillin, amoxicil- lin/clavulanic acid, streptomycin, tetracycline, doxycycline, nalidixic acid and sulfamethoxazole~ 95.5 ~of all isolates were multi-resistant strains~ Isolates from swine or cattle had more serious resistance than that of isolates from poultry. In conclusion, Salmonella isolates tested had diverse serotypes, and were resistant to different antimicrobials; The resistance surveillance should focus on the bacterium from animals which had long life cycles; Prudent use of antimicrobial must be abided in food-animal productions. Key words, food-animal; Salmonella ;identification; resistance