碳离子硅橡胶改性材料的表面性能检测及其细胞相容性评价

Surface properties and cell compatibility of carbon ion implanted silicone rubber

目的采用碳离子对硅橡胶(silicone rubber,SR)进行表面注入改性,并对改性材料进行形貌观察、理化性能检测及其细胞相容性评价。方法采用多功能离子注入机在不同碳离子注入量下对SR进行表面注入改性,得到碳-硅橡胶(Carbon ion implanted silicone rubber,C-SR),并采用扫描电镜(Scanning electron microscope,SEM)、傅立叶红外光谱(Fourier transform infrared spectrometer,FTIR)、X射线衍射(X-ray diffraction,XRD)技术对材料的表面性能进行检测。采用细胞增殖实验、免疫荧光及Western blot技术观察人真皮成纤维细胞在改性后材料表面的生长情况。结果 SEM发现各材料的表面形貌无明显差异,FTIR观察各材料的图谱无明显变化,XRD提示碳离子注入后形成了新的晶体结构,但差异并不显著。细胞增殖实验结果显示C-SR增殖能力较SR有明显提高(P<0.05)。免疫荧光及Western blot提示碳离子注入后,材料的细胞黏附性增加。结论采用碳离子注入制作的C-SR改性材料,与纯SR相比,表面性能和细胞相容性均得到了提高,有可能作为新的软组织填充材料。

Objective To evaluate the morphology, physicochemical properties and cell compatibility of silicone rubber （SR） after carbon ion being injected to its surface. Methods Different volumes of carbon ion injection was injected into the surface of SR with the aid of a multi-function ion injection machine. The obtained SR was named as carbon-silicone rubber（C-SR）. Scanning electron microscopy（SEM）, Fourier trans- form infrared spectrometry（FTIR） and X-ray diffraction （XRD） were used to analyze the surface properties of modified materials. Cell proliferation, immunofluorescenee staining and Western blot analysis were used to analyze the biocompatibility of modified materials after cocultured with human dermal fibroblasts. Results SEM and FFIR indicated that there was no any obvious difference in the morphology of the surface of modified C-SR. XRD showed that a new crystal structure was formed on the surface of the modified material after carbon-ion implantation, but the changes were not obvious compared with original materials. Cell proliferation experiment illustrated that human dermal fibroblasts grow better in C-SR than in SR （ P 〈 0. 05 ）. Immunofluorescence staining and Western blotting indicated that the expression of vinculin was enhanced in the C-SR materials, indicating that the carbon-ion implantation increased the cell adhesion. Conclusion Carbon-ion implantation to SR improves the surface properties and cell compatibility. C-SR might be used as a new filling material of soft tissue.