生长期内动态采收银杏叶中11种黄酮苷类成分的含量测定及季节变化分析(英文)

Simultaneous determination of eleven flavonoid glycosides in Ginkgo biloba leaves collected in different seasons by UPLC PDA method

本研究采用超高效液相色谱(UPLC)技术建立了银杏叶中11种黄酮苷类成分的含量测定方法,并对采集的2010年全年生长期内的北京产4株银杏叶样品进行测定。选用色谱柱为Agilent ZORBAX Eclipse Plus C18(50 mm×4.6 mm,1.8μm),流动相A为乙腈,流动相B为0.4%磷酸水溶液,梯度洗脱,检测波长为360 nm,流速为0.6 mL.min1。结果显示,11种黄酮苷在文中所述进样量范围内线性良好,平均加样回收率范围为97.85%～101.98%(RSD=0.75%～1.76%)。经方法学考察,所建立的方法精密度、稳定性、重复性、准确度良好,可准确反映银杏叶中各黄酮苷类成分的含量并初步分析季节动态变化趋势,为进一步了解银杏叶中黄酮苷类成分生物合成与积累的变化规律奠定了基础。

A new UPLC method was developed for the simultaneous determination of eleven characteristic flavonoid glycosides in Ginkgo biloba leaves.The natural occurrence of flavonoid glycosides in Ginkgo biloba leaves within one vegetative season was investigated for the first time.The analysis was performed on an Agilent ZORBAX Eclipse Plus C 18 column（50 mm × 4.6 mm,1.8 μm）,the mobile phase A was acetonitrile,the mobile phase B was 0.4% phosphate aqueous solution in a gradient elution at a flow rate of 0.6 mL.min 1,the detection was carried out at 360 nm.The result showed that eleven flavonoid glycosides had good linearity with good average recovery,separately.The method was proved to be accurate,rapid and good reproducible for the quality evaluation of Ginkgo biloba leaves,and provide an easy and rapid means for the quantitative analysis of flavonoid glycosides and their content fluctuation with seasons.