链脲佐菌素诱导C57BL／6小鼠发生糖尿病视网膜病变

Basic study of diabetic retinopathy in streptozotocin-induced diabetic C57BL/6 mice

目的研究糖尿病视网膜病变小鼠的眼底变化。方法给6周龄的C57BL/6小鼠腹腔注射链脲佐菌素(STZ)建立糖尿病模型。模型建立后5 w和10 w,分别行眼内压测量和视网膜电图(ERG)检测以及免疫荧光血管染色检查。结果与对照组小鼠相比较,糖尿病小鼠的眼压升高(P<0.01),ERG的a、b波振幅下降,免疫荧光染色可见糖尿病小鼠视网膜血管异常。结论糖尿病会导致小鼠眼底发生异常改变。这些病理改变可能是糖尿病视网膜病变的重要组成部分。

Objective To assess the retinal disease in diabetic mice. Methods six-week-old C57BL/6 mice were used as diabetic model with streptozotocin （STZ）. IOP and ERG were measured and retinal vascular was examined at the course of 5, 10 weeks after onset of diabetes. Results Compared with the control mice, the diabetic mice had significantly elevated IOP （P 〈 0.01 ） ; the amplitude of ERG a, b wave were significantly lower （P 〈 0. 01 ） and the retinal vasculars were abnormalities. Conclusions The retinal vascular lesion and ocu-lar hypertension are occured in diabetic mice. These pathological changes may be an important component of diabetic retinopathy （DR）.