延迟增强核磁共振评估经皮球囊封堵冠状动脉法制备的小型猪心肌梗死模型

Delayed-enhanced magnetic resonance imaging for assessing a minipig myocardial infarction model established by percutaneous balloon occlusion

目的利用延迟增强核磁共振(DE-MRI)评估经皮球囊封堵冠状动脉法制备小型猪心肌梗死模型。方法麻醉后,经小型猪右侧股动脉置入2.0 mm×15.0 mm球囊至左前降支(LAD)中远段,完全封堵血流90 min。DE-MRI评估心梗后梗死面积及心功能变化,病理检查进一步明确结果。结果 DE-MRI显示平均心肌梗死面积为10.2±2.9 cm3。与对照组比较,急性心肌梗死组心输出量、左室射血分数和心搏量均明显下降(P<0.001);而左室舒张末容积和左室收缩末容积均升高(P<0.05)。DE-MRI结果和左室短轴病理切片相对应。结论建立了有效的评估小型猪急性心肌梗死模型,适用于评价干细胞移植治疗急性心肌梗死。

Objective To assess a minipig model of acute myocardial infarction （AMI） established by percutaneous balloon occlusion using delayed-enhanced magnetic resonance imaging （DE-MRI）. Methods A minipig model of AMI was established by placement of a 2.0 mm × 15.0 mm percutaneous transluminal coronary angioplasty balloon in the middle left anterior descending artery （LAD） through a percutaneous femoral puncture in the right inguinal region. The left anterior descending coronary artery （LAD） was occluded for 90 min, followed by assessment of the infarct size and cardiac function with DE-MRI, and the results were confirmed by pathological examination. Results DE-MRI showed a mean infarcts size of 10.2＋_2.9 cm3 in the minipig models. Compared to the control group, the minipigs with AMI had significantly increased end-diastolic and end-systolic volumes （P〈0.05） with a decreased stroke volume, ejection fraction and cardiac output （P〈0.001）. These DE-MRI values were matched with the microsphere values obtained from short-axis slices in pathological examination. Conclusion We have established a feasible approach for evaluating minipig models of AMI as a platform for assessing the therapeutic effect of stem cell transplantation for AMI.