摘要
目的建立中波紫外线照射诱导大鼠发生白内障的实验方法,应用聚丙烯酰胺凝胶电泳法(sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE)及高效液相色谱法分析紫外线照射致大鼠晶状体相关蛋白表达谱的变化。方法利用中波紫外线分别照射大鼠0d、1d、3d、7d,建立实验性白内障大鼠模型,并应用SDS-PAGE检测晶状体总蛋白的表达情况以及高效液相色谱法分析晶状体相关蛋白表达谱的变化。结果与未经紫外线照射的大鼠晶状体相比,紫外线照射大鼠1d后,晶状体透明度无明显变化,照射3d后开始出现轻度晶状体混浊,7d后可形成皮质性白内障。应用SDS-PAGE检测,经紫外线照射后晶状体总蛋白表达量有所改变;高效液相色谱法检测晶状体相关蛋白表达谱的变化,发现随照射时间的延长,晶状体相关蛋白中有1种蛋白下调表达,4种蛋白为先上调后下调表达,2种蛋白为先下调后上调表达,2种蛋白表达无规律。结论成功应用中波紫外线照射方法制作了白内障大鼠模型;紫外线照射可使晶状体相关蛋白表达发生改变,变化趋势呈多样性。
Objective To explore the experimental method of establishing cata- ract-rat induced by ultraviolet B （LWB） radiation, and investigate the changes of rele- vant protein profile of lens in rats induced by UVB via SDS-PAGE and high performance liquid chromatography （HPLC）. Methods The experimental rat cataract model was established through midwave ultraviolet B radiation for o day,1 day,3 days and 7 days. Meanwhile,the changes of lens protein profiles induced by UVB in rats via SDS-PAGE and HPLC assays were also examined. Results Compared with lens transparency of rats without UVB irradiation, it was found that no obvious change occurred after UVB radiation for 1 day in rats. However, the mildly turbid lens occurred after irradiation for 3 days, and cortical cataract was observed after LWB irradiation for 7 days in rats. The results of SDS-PAGE indicated that total lens proteins expression changed after UVB ir- radiation. The results of HPLC showed one protein expression was continuously de- creased and 4 proteins expression were firstly up-regulated and then down-regulated, whereas 2 proteins expression were initially down-regulated and then up-regulated, 2 proteins expression were unchanged with the irradiation prolonged. Conclusion The rat cataract model is successfully established via UVB radiation, and UVB radiation can lead the change of lens protein profile with diversity.
出处
《眼科新进展》
CAS
北大核心
2013年第1期17-21,共5页
Recent Advances in Ophthalmology
基金
山东省自然科学基金资助(编号:ZR2010HM032)~~
