摘要
目的:观察外源性肾上腺髓质素(AM)对卵巢癌细胞HO8910迁移的影响,并探讨其作用机制。方法:外源性应用AM后,采用划痕实验观察卵巢癌细胞系HO8910的迁移功能,并且利用蛋白质印迹法检测ERK1/2及p-ERK1/2蛋白的表达,以了解AM对ERK1/2蛋白及其活性的影响。结果:外源性给予AM(100nmol/L)的细胞12h迁移率为(62.61±4.51)%,阴性对照细胞的迁移率为(29.23±4.15)%,AM可以促进卵巢癌细胞HO8910的迁移,P=0.001。提前应用ERK1/2抑制剂PD98059的细胞在外源性AM(100nmol/L)的刺激下迁移率为(37.97±3.44)%,比单纯应用AM刺激的细胞迁移率降低,P=0.002。外源性给予AM(100nmol/L)可以促进卵巢癌细胞的ERK1/2蛋白磷酸化。结论:AM促进卵巢癌细胞的迁移,与细胞的ERK1/2活化相关,可能为卵巢癌迁移的治疗提出一个新的研究方向。
OBJECTIVE: To investigate the exogenous adrenomedullin (AM) effection in ovarian cancer cell HO8910 migration and its mechanisms. METHODS: The migration of HO8910 was examined by wound healing test. The expression levels of ERK1/2 and its phosphorylation pattern were examined by western blot. RESULTS: The negative control cells wound healing rate was(29.23士4.15)%. The wound healing rate of cells with exogenous AM (100 nmol/L) was (62.61士4.51)%. AM could promote the motility of HO8910(P=0. 001). Using PD98059,the inhibitor of ERK1/2 one hour before AM,the ceils wound healing rate was(37.97士3.44)% ,so PD98059 could inhibit the AM function in HO8910 (P=0. 002). The phosphorylation of ERK1/2 was significantly increased by AM. CONCLUSION: The AM may play im- portant roles in ovarian epithelial cancer cell migration via phosphorylation of ERK1/2, and may be treated as a new target of ovarian epithelial cancer migration.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第22期1709-1711,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
辽宁省自然科学基金(2009225035)