肾上腺髓质素对卵巢癌细胞迁移影响及其机制的探讨

Mechanism of migration promotion by adrenomedullin in ovarian cancer cell

目的:观察外源性肾上腺髓质素(AM)对卵巢癌细胞HO8910迁移的影响,并探讨其作用机制。方法:外源性应用AM后,采用划痕实验观察卵巢癌细胞系HO8910的迁移功能,并且利用蛋白质印迹法检测ERK1/2及p-ERK1/2蛋白的表达,以了解AM对ERK1/2蛋白及其活性的影响。结果:外源性给予AM(100nmol/L)的细胞12h迁移率为(62.61±4.51)%,阴性对照细胞的迁移率为(29.23±4.15)%,AM可以促进卵巢癌细胞HO8910的迁移,P=0.001。提前应用ERK1/2抑制剂PD98059的细胞在外源性AM(100nmol/L)的刺激下迁移率为(37.97±3.44)%,比单纯应用AM刺激的细胞迁移率降低,P=0.002。外源性给予AM(100nmol/L)可以促进卵巢癌细胞的ERK1/2蛋白磷酸化。结论:AM促进卵巢癌细胞的迁移,与细胞的ERK1/2活化相关,可能为卵巢癌迁移的治疗提出一个新的研究方向。

OBJECTIVE： To investigate the exogenous adrenomedullin （AM） effection in ovarian cancer cell HO8910 migration and its mechanisms. METHODS： The migration of HO8910 was examined by wound healing test. The expression levels of ERK1/2 and its phosphorylation pattern were examined by western blot. RESULTS： The negative control cells wound healing rate was（29.23士4.15）%. The wound healing rate of cells with exogenous AM （100 nmol/L） was （62.61士4.51）%. AM could promote the motility of HO8910（P=0. 001）. Using PD98059,the inhibitor of ERK1/2 one hour before AM,the ceils wound healing rate was（37.97士3.44）% ,so PD98059 could inhibit the AM function in HO8910 （P=0. 002）. The phosphorylation of ERK1/2 was significantly increased by AM. CONCLUSION： The AM may play im- portant roles in ovarian epithelial cancer cell migration via phosphorylation of ERK1/2, and may be treated as a new target of ovarian epithelial cancer migration.