肾透明细胞癌组织E-cadherin和β-catenin及Cyclin D1表达的研究

Expressions of E-cadherin,β-catenin and Cyclin D1 in renal clear cell carcinoma

目的:探讨E-cadherin、β-catenin及Cyclin D1在肾透明细胞癌(RCCC)中的表达及意义。方法:用免疫组化法检测E-cadherin、β-catenin及Cyclin D1在67例RCCC组织中的表达情况,并分析其与预后的关系。结果:E-cadherin及Cyclin D1在RCCC组织中表达率分别为35.8%(24/67)和44.8%(30/67)。β-catenin在RCCC组织中胞膜和胞质表达率分别为23.9%(16/67)和68.7%(46/67)。E-cadherin在RCCC组织中的表达与肿瘤病理分级及临床分期相关(r=0.393,P=0.001;r=0.365,P=0.002)。E-cadherin与β-catenin胞膜表达之间具有显著相关性,r=0.531,P=0.000。β-catenin在RC-CC组织中胞膜表达与肿瘤临床分期相关,r=0.386,P=0.001。Cyclin D1与β-catenin胞膜表达之间具有显著相关性,r=0.481,P=0.000。Cyclin D1在RCCC中的表达与肿瘤体积大小具有相关性,r=0.325,P=0.007。结论:E-cadherin和β-catenin蛋白低表达或异常表达与RCCC的病理分级和临床分期有关,并对RCCC预后评价有一定参考意义。

OBJECTIVE：To investigate the expression and significance of E-cadherin,β-catenin and Cyclin D1 in renal clear ceil carcinoma （RCCC）. METHODS：The Expression of E-cadherin,β-eatenin and Cyclin D1 in 67 cases of RCCC was detected by immunohistochemistry. The relation between the expressions and prognosis was analyzed. RESULTS： Positive rates of E-cadherin and Cyclin D1 were 35.8% （24/67） and 44.8% （30/67） in RCCC respectively. Membrane and cytoplasm positive rates of i3-catenin were 23.9 % （16/67） and 68.7 % （46/67） respectively. The expression of E-cadherin was correlated with tumor pathologic grading and clinical staging （r： 0. 393, P = 0. 001 ; r= 0. 365, P： 0. 002）. The expression rate of E-cadherin was correlated with that of β-catenin in membrane （r=0. 531 ,P：0. 000）. The membrane expression of β-catenin was correlated with tumor clinical staging （r：0. 386 ,P=0. 001）. The expression rate of Cyclin D1 was correlated with that of β-catenin in membrane （r： 0. 481, P=0. 000）. The expression rate of Cyclin D1 was correlated with tumor size （r= 0. 325, P = 0. 007）. CONCLUSIONS： The results suggest a significant relationship between E-cadherin, β-catenin protein low expression or abnormal expression of RCCC and tumor pathologic grading or clinical staging. E-cadherin,β-catenin may be a useful prognostic indicator in RCCC.