小儿肾母细胞瘤组织SOCS-3蛋白表达及其临床意义分析

Expression and the clinical significance of SOCS-3 in nephroblastoma tissues

目的:研究细胞因子信号转导抑制因子-3(SOCS-3)蛋白在小儿肾母细胞瘤组织中的表达及意义。方法:应用组织微阵列技术结合免疫组化SP法检测58例肾母细胞瘤标本和17例瘤旁对照组织中的SOCS-3的表达状况,并分析其临床病理意义。结果:肾母细胞瘤组织中仅14例SOCS-3呈弱阳性表达,表达率为24.1%(14/58);17例瘤旁组织中SOCS-3蛋白阳性表达率为88.2%(15/17),差异有统计学意义,P<0.01。SOCS-3在肾母细胞瘤组织的表达随着肿瘤的临床分期增加而降低(P<0.05),与肾母细胞瘤的临床分期呈负相关,P<0.01;在组织结构良好型与组织结构不良型SOCS-3蛋白表达(47.6%、10.8%)间差异有统计学意义,P<0.05。结论:SOCS-3的表达下降可能与肾母细胞瘤的发生发展密切相关。

OBJECTIVE：To investigate protein expression of SOCS-3 and its clinacal significance in Nephroblastoma. METHODS： Using tissue microarray combination with immunohistochemical measure to investigate the protein expression status of SOCS-3 in 58 cases of Nephroblastoma and 17 cases of adjacent tumor samples,analyzing the relation of expression status of SOCS-3 with clinicopathological factors. RESULTS：The ratio of SOCS-3 protein expression was increased in nephroblastoma tissues 24.1 % （ 14 / 58） compared to adjacent tumor cases 88.2 % （ 15 / 17, P〈0.01 ） by using streptavidin- biotin pemixidase （SP） method. Within the specimens of Pca group, the differences were statistically significant among pathological grade and clinical stage （P〈0.05）. There was statistically significant among FHand LH （P〈0. 05）. There was a negative correlation between the expression of SOCS-3 and clinical grades （P〈0.01）. CONCLUSION： The low expressions of SOCS-3 may have a role in the development and progress of Nephroblastoma.