前列腺癌组织Clusterin与p27及Survivin表达临床意义的研究

Clinical significance of the expressions of Clusterin,p27 and Survivin in prostate cancer tissues

目的:探讨凋亡相关蛋白Clusterin、p27和Survivin在前列腺癌组织中的表达及临床意义。方法:应用免疫组织化学方法检测68例前列腺癌组织和47例前列腺增生组织Clusterin、p27和Survivin蛋白的表达。结果:前列腺癌组织Clusterin、p27和Survivin蛋白的阳性表达率分别为75.0%(51/68)、72.1%(49/68)和67.6%(46/68);前列腺增生组织Clusterin、p27和Survivin蛋白的阳性表达率分别为25.5%(12/47)、19.1%(9/47)和23.4%(11/47)。Clusterin、p27和Survivin蛋白阳性表达率在前列腺癌组织和前列腺增生组织中差异有统计学意义,P<0.01。Clusterin、p27和Survivin蛋白表达与前列腺癌病理分级、临床分期和转移情况具有相关性,P<0.05。前列腺癌组织Survivin与Clusterin、Sur-vivin与p27的表达呈正相关,P<0.01。结论:联合检测Clusterin、p27和Survivin蛋白有助于对进展性前列腺癌细胞的侵袭力作出正确评价,以指导临床治疗。

OBJECTIVE：To detect the expressions of Clusterin,p27,Survivin and their correlation in prostate cancer （PCa） tissues. METHODS： Immunohistochemistry was employed to measure the expression of Clusterin, p27 and Survivin in prostatic cancer and 47 cases benign prostatic hyperplasia. RESULTS： In prostatic cancer tissue,the positive expression rates of Clusterin,p27 and Survivin were 75.0% （51/68）,72.1 % （49/68） and 67.6% （46/68）. In benign prostatic hyperplasia tissue the positive expression rates of Clusterin, p27 and Survivin were 25. 5% （12/47）, 19. 1% （9/47）, 23.4% （11/47）. The positive expression of clusterin,p27 and Survivin between prostatic cancer and benign prostatic hyperplasia were significantly different （P〈0.01）. The positive expression of Clusterin, p27 and Survivin were related with the histologic grade, clinical stage and metastasis of prostatic cancer （P〈0.05）. The expression of Survivin was a positive correlation with clusterin and p27 expression in prostatic cancer （P〈0.01）. CONCLUSION： The detection of Clusterin, p27 and Survivin protein in PCa may help to evaluate the degree of cell apoptosis and decide the therapeutic strategies.