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环介导等温扩增技术及其在病原微生物检测中的应用 被引量：3

Loop-mediated isothermal amplification technology and its application in pathogenic microorganism detection
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摘要 环介导等温扩增(LAMP)技术是近年发展起来的一项新的快速核酸恒温扩增技术,具有高效、快速、特异、易检测、易操作等特点,自面世以来被科学家认为是能替代常规PCR的一项扩增技术,非常适用于现场检测和基层检测。本文就LAMP技术的原理、特点、进展及其在病原微生物检测中的应用进行了简要的概述。 Loop-mediated isothermal amplification（LAMP） technology is a novel nucleic acid isothermal amplification technology developed in recent years.Because of its high effiency,rapidity,specificity,and simplicity,scientists believe it will be a routine amplification technology replacing the conventional PCR method.This paper briefly reviews the principle of the technology,techinical characteristics,progress,and its application in pathogenic microorganism detection.
作者 肖维威 周琳华 郑文岭 马文丽
机构地区 南方医科大学基因工程研究所
出处 《基础医学与临床》 CSCD 北大核心 2013年第1期19-23,共5页 Basic and Clinical Medicine
关键词 环介导等温扩增 检测 病原体 loop-mediated isothermal amplification detection pathogenic microorganism
分类号 R446.5 [医药卫生—诊断学]
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参考文献29

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二级参考文献27

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共引文献37

同被引文献59

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引证文献3

二级引证文献5

基础医学与临床
2013年 第1期

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