5-氮-2'-脱氧胞苷逆转膀胱癌细胞hepaCAM基因的表达及对其生长的抑制

5-aza-2′-deoxycytidine reverses the expression of hepaCAM mRNA and inhibits the growth of T24 and BIU-87 cells

目的研究5-氮-2-脱氧胞苷(5-aza-CdR)逆转hepaCAM表达及抑制膀胱癌细胞的生长。方法 MTT法检测细胞增殖;流式细胞术检测细胞凋亡;RT-PCR检测hepaCAM mRNA的表达。结果在T24细胞中,3.0和10.0μmol/L5-aza-CdR药物浓度的抑制率明显高于0.3和1.0μmol/L 5-aza-CdR(P<0.05),在BIU-87细胞中,5.0μmol/L5-aza-CdR药物浓度的抑制率明显高于0.1和0.5μmol/L(P<0.05),并且药物处理细胞72 h的抑制率明显高于24和48 h(P<0.05);5-aza-CdR处理细胞后,T24和BIU-87细胞凋亡数明显增加(P<0.05);并可逆转hepaCAMmRNA表达。结论 5-aza-CdR可使hepaCAM基因重新表达,并通过诱导凋亡而抑制膀胱癌细胞的生长。

Objective To investigate the effect of 5-aza-CdR on expression of hepaCAM mRNA and the inhibition of T24 and BIU-87cell lines.Methods The effects of 5-aza-CdR on cell proliferation of T24 and BIU-87 cell lines were measured by MTT;Flow cytometry was used to examine apoptosis of T24 and BIU-87 cells;RT-PCR was used to detect the expression of hepaCAM in T24 and BIU-87 cell lines with or without 5-aza-CdR treatment.ResultsThe inhibition ratios of 3.0 and 10.0 μmol/L 5-aza-CdR were significantly higher than that of 0.3 and 1.0 μmol/L 5-aza-CdR in T24 cell（P0.05）.The inhibition ratio of 5.0 μmol/L 5-aza-CdR was higher than that of 0.1 and 0.5 μmol/L 5-aza-CdR in BIU-87 cell（P0.05）.Cell apoptotic rate increased in cells with 5-aza-CdR treatment（P0.05）.The expression of hepaCAM mRNA was reversed in T24 and BIU-87 cells after treatment.Conclusions 5-aza-CdR can reverse the expression of hepaCAM mRNA and therefore inhibit the cell growth through inducing apoptosis in bladder cancer tissue.