四川主要松树内生拟盘多毛孢

Endophytic Pestalotiopsis from the main pine species in Sichuan

从5种松树健康的枝、叶、干中共分离了2,400个组织块,得到199株内生拟盘多毛孢Pestalotiopsis,形态学和分子生物学鉴定分属于11个种,平均分离率为8.29%。所得11种内生拟盘多毛孢都是首次在四川的松属植物中分离得到,其中葡萄生拟盘多毛孢P.uvicola和山桃草拟盘多毛孢P.gaurae为首次在松树上分离得到。基于ITS序列的分子系统发育分析显示松树内生拟盘多毛孢种类之间存在一定的遗传差异,部分内生拟盘多毛孢与病原拟盘多毛孢的ITS序列具有很高的同源性。

One hundred and ninety-nine isolates of endophytic Pestalotiopsis were isolated from 2,400 tissue masses of healthy branches, leaves and stems of five species of pine in Sichuan, and 11 species were identified based on morphological characteristics and ITS sequences. The average isolation rates of endophytic Pestalotiopsis was 8.29%. P uvicola and P gaurae were dominant among the 11 species, which were firstly recorded on pines in Sichuan. Molecular phylogenetic analysis based on ITS sequences indicated that there were some genetic differences among these endophytic Pestalotiopsis species, and part of them were highly homologeneous with the pathogenic Pestalotiopsis.