依达拉奉对Alzheimer病模型大鼠认知功能及海马内乙酰胆碱含量的影响

Effect of Edaravone on cognitive function and level of acetylcholine in hippocampus in rat models of Alzheimer disease

目的探讨依达拉奉(EDA)对Alzheimer病(AD)模型大鼠认知功能及海马内乙酰胆碱(Ach)含量的影响。方法 30只大鼠随机分为假手术组、AD组及EDA组。向AD组及EDA组大鼠海马内立体定向注射β淀粉样蛋白(Aβ)1-40建立AD模型;EDA组术后给予EDA腹腔注射共2周。术后第5周进行Morris水迷宫试验评价各组大鼠的认知功能,高效液相色谱法测定各组大鼠海马区Ach含量。结果与假手术组比较,EDA组及AD组水迷宫试验中的逃避潜伏期明显延长,目的象限停留时间明显缩短,穿环次数明显减少(P<0.05～0.01);与AD组比较,EDA组逃避潜伏期明显缩短,目的象限停留时间明显增加,穿环次数明显增多(P<0.05～0.01)。与假手术组比较,EDA组及AD组海马区Ach含量明显减少(均P<0.01);EDA组海马区Ach含量明显较AD组明显增加(P<0.01)。结论 EDA通过增加AD模型大鼠海马Ach含量,改善其认知功能。

Objective To explore the effect of Edaravone（EDA） on cognitive function and level of acetylcholine（Ach） in hippocampus in rat models of Alzheimer disease（AD）.Method Thirty rats were randomly divided into sham operated group,AD group and EDA group.Amyloid-β-protein（Aβ） 1-40 was injecting into the rat＇s hippocampus of AD and EDA groups to establish AD model.Then,EDA was intraperitoneally injected in EDA group for 2 weeks.After 5 weeks of the operation,the cognitive function of rats in each group was measured by Morris water maze test;and the level of Ach in hippocampus was detected by high performance liquid chromatography.Results Compared with sham operated group,the escape latencies in EDA and AD groups were obviously prolonged,purpose quadrant retention times were significantly shorter,crossing circle times were significantly increased（P0.05-0.01）.Compared with AD group,the escape latency in EDA group was significantly shorter,purpose quadrant retention time was significantly shortened,crossing circle times was significantly increased（P0.05-0.01）.Compared with sham operated group,the levels of Ach in hippocampus in EDA and AD groups were obviously decreased（all P 0.01）;but which in EDA group was obviously increased than AD group（P0.01）.Conclusion EDA can improve cognitive function in rat models of AD by increase the level of Ach in hippocampus.