口咽部鳞癌中人乳头瘤病毒感染的检测

Detection of Human Papillomavirus in Oropharyngeal Squamous Cell Carcinoma

目的检测口咽鳞癌组织标本人乳头瘤病毒(HPV)感染情况,获得其型别分布及肿瘤临床病理特点。方法选择1999年1月至2009年12月在中国医学科学院肿瘤医院病理科存档的满足条件的口咽鳞癌活检或手术标本共66例,采用SPF10-DNA LiPA分型方法进行检测。结果 66例标本中,11例(16.7%)检测出HPV,其中HPV-16型7例,HPV-16/11型1例,HPV-35、PV-58/52和HPV-33/52/54型各1例,HPV-16型占所有感染者的比例为72.7%(8/11)。HPV阳性标本中,女性(36.4%比1.8%,P=0.002)、非吸烟(36.4%比0,P=0.001)及非饮酒者(45.5%比1.8%,P=0.001)所占的比例均明显高于HPV阴性标本;低分化所占为81.8%,与HPV阴性标本的63.7%比较,差异无统计学意义(P=0.409);HPV阳性和阴性标本在T分级、N分级及临床分期方面差异均无统计学意义(P均>0.05)。结论口咽鳞癌患者HPV感染率为16.7%,HPV相关口咽鳞癌具有独特的流行病学和临床病理特点。

Objective To investigate the infection rate and subtypes of human papilloma virus（HPV） in patients with oropharyngeal squamous cell carcinoma（OSCC） and analyze the clinicopathologic features of patients with or without HPV infection.Methods A total of 66 biopsy or surgical specimens of OSCC archived in the Pathology Department of Chinese Academy of Medical Sciences were analyzed by polymerase chain reaction（PCR）,and the generic amplification products were detected by DNA enzyme immunoassay（DEIA） and typed by reverse hybridization line probe assay.Results HPV-DNA was detected in 11（16.7%） of all specimens.Among them,7 were infected with HPV-16,and the remaining 4 patients were infected with HPV-16/11,HPV-35,HPV-58/52,and HPV-33/52/54,respectively.HPV-16 was detected in 72.7% of all positive specimens.There were more females in HPV-positive group than HPV-negative group（36.4% vs.1.8%,P=0.002）.Patients with HPV-positive tumors were more likely to be non-smokers（36.4% vs.0,P=0.001） and non-drinkers（45.5% vs.1.8%,P=0.001） than those with HPV-negative tumors.The proportion of moderately or poorly differentiated tumors was higher in HPV-positive patients than HPV-negative patients（81.8% vs.63.7%）,although without statistical significance（P=0.409）.No difference was observed in T classification,N classification,and overall tumor stage.Conclusions HPV infection rate was 16.7% in this cohort.HPV-positive OSCC has its unique etiologic and clinicopathological characteristics.