HLA—G14bp基因多态性及血浆sHLA—G水平与儿童EV71感染的关系研究

Association of HLA-G 14bp gene polymorphisms and plasma sHLA-G level with susceptibility toEV71 infection in children

目的探讨人类白细胞抗原G（HLA—G）14bp插入／缺失多态性及血浆可溶性HLA—G（sHLA—G）水平与儿童肠道病毒71型（EV71）感染的相关性。方法采用聚合酶链反应结合聚丙烯酰胺凝胶电泳（PCR—PAGE）技术，对125例EV71感染重症手足口病（HFMD）患儿及133例正常对照儿童进行HLA—G14bp插入／缺失多态性的检测；采用酶联免疫吸附测定（ELISA）检测其中66例重型和15例危重型EV71感染HFMD患儿及133例正常对照儿童血浆中sHLA-G水平。结果EV71感染重症HFMD组HLA—G14bp插入／缺失基因型多态性14bp-／-、14bp＋／-和14bp＋／＋的频率分别为49．6％、42．4％和8．0％，正常对照组分别为34．6％、48．9％和16．5％，两组基因型多态性分布频率差异有统计学意义（X2=7．850，P=0．020）；两组等位基因分布频率差异有统计学意义（X2=7．830，P=0．005，EV71感染重型组血浆sHLA—G水平明显高于正常对照组（Z=-9．692，P=0．000），危重型组血浆中sHLA—G水平明显高于重型组（Z=-2．420，P=0．016）。结论HLA—G14bp插入／缺失基因型多态性与儿童EV71感染有关联，血浆sHLA—G水平可作为EV71感染的辅助诊断指标。

Objective Explore the relationship between the HLA-G 14bp insertion/deletion polymorphism and the infection of Enterovirus 71 （EV71） for children. Methods We genotyped HLA-G 14bp insertion/deletion polymorphism of 125 severe HFMD children infected with EV71 and 133 normal controls by PCR-PAGE;detected the plasma sHLA-G level of 66 heavy type and 15 critical type and 133 normal controls by ELISA. Results Frequencies of the genotype 14 bp - / - , 14 bp ＋ / - and 14 bp ＋ / ＋ were 49.6% , 42.4% and 8.0% for the severe HFMD children infected with EVT1, and 34. 6% ,48.9% and 16.5% for the normal controls, respectively. A significant difference was observed for the frequencies of the HLA-G 14bp genotype between the two groups（x2 = 7. 850,P = 0. 020）. And for the allele frequencies. The plasma sHLA-G levels in heavy type were dramatically higher than that in normal controls（ Z = -9. 692, P =0. 000）. The plasma sHLA-G levels in children with critical HFMD were dramatically higher than that with heavy type（Z = - 2.420,P = 0. 016）. Conclusion There was a relationship between the HLA-G 14 bp insertion/deletion polymorphism and the susceptibility to the severe HFMD children infected with EV71 and the plasma sHLA-G might be considered as a index for auxiliary diagnosis the severe HFMD infected with EV71.