湖北省HIV-1流行株gag基因序列测定及亚型分析

Subtype and sequence analysis of the gag genes for HIV-1 strains isolated in Hubei province

目的了解湖北省HIV-l感染者流行毒株亚型分布和流行趋势。方法随机采集湖北省HIV-1感染者的抗凝全血标本，分离血浆，提取病毒RNA，用套式聚合酶链反应扩增HIV．1病毒gag基因，并进行序列测定和亚型分析。结果对80份HIV-l感染者的样品进行扩增，得到了62份样品的HIV-1基因片段。共发现7种HIV-1亚型和流行重组株。泰国B’亚型占11．3％（7／62），欧美B亚型占4．8％（3／62），G亚型占4．8％（3／62），CRF07-BC占22．6％（14／62），CRF08-BC占6．5％（4／62），CRF01-AE占48．4％（30／62），CRFl5-01B占1．6％（1／62）。在湖北省发现了CRFl5-01B和G亚型。结论湖北省存在多种HIV-1亚型和流行重组型，CRF01-AE、CRF07．BC两种重组亚型毒株为目前湖北省HIV-1流行优势毒株，应加强对HIV—I毒株亚型变异的监测，及时调整防治策略。

Objective To investigate the characteristic of subtypes and genetic diversity of HIV-1 circulating in Hubei province and its molecular epidemiological linkages with regard to risk factors of viral transmission. Methods plasma samples of 80 diagnosed individuals was characterized. The gene fragments of gag were amplified by reverse transcriptase polymerase chain reaction （RT-PCR） and HIV-1 genotypes were determined based on the nucleotide sequences of gag region. Results Seven HIV-1 group M subtypes or CRF including B, B＇, G, CRFO1-AE, CRF07-BC, CRF08-BC and CRF15-O1B were identified. CRF01- AE was found to be the most dominant subtype （48.4%） followed by CRFT-BC （22.6%） and B＇ （12.9%）. Conclusion The data from this study indicate the existence of multiple HIV-1 subtypes or CRFs in Hubei province and the surveillance of HIV-1 gene variation should be paid more attention to.