正常与退变髓核细胞的变形能力

Distortion capability of normal and degenerative nucleus pulposus cells

背景:细胞的变形能力在较大程度上能反映出细胞结构与功能的改变,目前对髓核细胞在细胞力学方面的研究较少。目的:分析正常和退变髓核细胞的变形能力。方法:正常髓核组织和退变髓核组织分别取材于3例脊柱侧弯患者和3例椎间盘突出患者术中取出的废弃髓核组织,用胰蛋白酶和Ⅱ型胶原酶分离提取细胞,行原代培养,通过微管加载装置适宜负压下测量细胞在第4秒时吸入微管的长度,并行统计学处理。结果与结论:正常髓核细胞和退变髓核细胞所用的负压分别为(411.31±27.93)Pa,(434.24±46.26)Pa,差异有显著性意义(P<0.05)。在4s时吸入长度分别为(2.20±0.92)μm,(2.48±0.71)μm,差异无显著性意义(P>0.05)。结果可见在规定时间的情况下,吸入微管的长度基本相同时,退变髓核细胞所需要的负压较正常髓核细胞大,说明退变髓核细胞的变形能力比正常髓核细胞差。

BACKGROUND：The distortion capability of cells can reflect the changes of cell structure and function.To date,the studies on the cell mechanics of nucleus pulposus are rare.OBJECTIVE：To analyze the distortion capability of normal and degenerative nucleus pulposus in vitro.METHODS：The normal and degenerative nucleus pulposus cells were respectively obtained from discarded nucleus pulposus tissue of 3 scoliosis patients and 3 intervertebral disc hernitaion patients.Pancreatin and collagenase type Ⅱ were used to isolate the nucleus pulposus tissue and performed with primary culture.The micropipette aspiration test was used to measure the length of nucleus pulposus cells aspirated into micropipette at 4 seconds under suitable negative pressure,and the statistical analysis was performed.RESULTS AND CONCLUSION：The negative pressure of the normal and degenerative nucleus pulposus cells were（411.31±27.93） Pa and（434.24±46.26） Pa respectively,and the difference was significant（P 0.05）.Within 4 seconds,the lengths of the normal and degenerative nucleus pulposus cells aspirated into micropipette were（2.20±0.92） μm and（2.48±0.71） μm,their differences were not significant（P 0.05）.With the certain time and the same length of the normal and degenerative nucleus pulposus cells aspirated into micropipette,the degenerative nucleus pulposus cells needed greater negative pressure than normal nucleus pulposus cells.It indicates that the distortion capability of degenerative nucleus pulposus cells is worse than that of normal nucleus pulposus cells.