尼莫地平对兔早期脊髓缺血再灌注损伤的保护作用

The protective effects of Nimodipine against the ischemia-reperfusion injury during the early stage of spinal cord-ischemia in the rabbits

目的探讨尼莫地平对兔早期脊髓缺血再灌注损伤后出现的神经功能障碍和脊髓组织病理变化的保护作用。方法将18只新西兰大白兔随机分为假手术组(n=6)、对照组(n=6)和尼莫地平组(n=6),按照Kwun等的方法建立兔脊髓缺血再灌注损伤模型,术后早期尼莫地平组给予尼莫地平治疗,对照组和假手术组给予等剂量生理盐水,并于每次给药前按照Reuter方法评估兔感觉运动功能。3d后取兔腰段(L5～L7)脊髓行病理学HE染色、尼氏染色和免疫组化染色进行分析。结果假手术组、对照组、尼莫地平组1～3 d内Reuter评分分别为[0.00(0.00,0.00),5.50(5.50,6.13),5.25(5.00,6.00)],[0.00(0.00,0.00),5.50(5.50,6.13),5.50(5.38,5.63)]和[0.00(0.00,0.00),6.00(6.00,6.63),5.50(5.00,6.00)]。其中1 d和2d时对照组和尼莫地平组之间差异无统计学意义(P>0.017),而3 d时三组之间两两比较差异均有统计学意义(P<0.017);病理学染色结果表明三组脊髓灰质前角尼氏小体计数和神经胶质原纤维酸性蛋白染色指数分别为[27.50(25.75,30.25),2.00(1.00,2.25),4.50(4.00,6.00)]和[1.00,2.00);13.00(12.00,14.50),4.50(3.75,5.25)],三组之间两两比较差异均有统计学意义(P<0.017)。结论尼莫地平对早期脊髓缺血再灌注损伤有一定程度的保护作用。

Objective To investigate the protective effects of Nimodipine against the ischemia-reperfusion in- jury during the early stage of spinal cord-ischemia in rabbits. Methods Eighteen New Zealand white rabbits were randomly divided into the sham operation group （n = 6）, the control group （n = 6） and the Nimodipine group （n = 6）. The spinal cord ischemia-reperfusion injury was induced in rabbits according to the method of Kwun. Nimodipine group received Nimodipine, while the control and sham operation groups received an equal dose of saline after is- chemia. Reuter was used to evaluate sensory/motor function of the rabbits. The HE staining, Nissl staining and im- munohistochemical staining were used to assess the pathology of the lumbar spinal cord （L5 - L7）. Results The Reuter scores in the sham operation group, the control group and the Nimodipine group at day 1, day 2 and day 3 were 0.00 （0.00, 0.00）, 5.50 （5.00, 6.13） and 5.25 （5.00, 6.00） in sham group, 0.00（0.00, 0.00）, 5.50 （5.50, 6.13） and 5.50 （5.38, 5.63） in operation group and 0.00（0.00, 0.00）, 6.00（6.00, 6.63）, and 5.50 （5.00, 6.00） in the Nimodipine group. There were significant differences in Reuter scores between the control group and Nimodipine group at day three but not at day one and day two （P 〈 0.017, P 〉 0.017, P 〉 0.017） The num- bers of Nissl stained neurons in the anterior horn of the spinal cord gray matter were 27.50 （25.75, 30.25）, 2.00（1.00, 2.25） and 4.50 （4.00, 6.00） in sham, control and Nimodpine groups, respectively. The Glial fibrillary acidic protein （GFAP） in the anterior horn of the spinal cord gray matter group were 1.00 （1.00, 2.00）, 13.00 （12.00, 14.50） and 4.50 （3.75, 5.25） in in sham, control and Nimodpine groups, respectively. There were statistically significant differences in Nissl stained neurons and GAFP between any two groups of the three groups （P 〈 0.017）. Conclusion Nimodipine has protective effects against the early spinal cord ischemia-reperfusion injury.