托烷司琼不同途径和不同剂量给药对顺铂引起的大鼠异嗜高岭土行为的影响

The effects of different tropisetron administration routes and dosages on cisplatin-induced kaolin intake in rats

目的比较鞘内和静脉内两种不同途径给不同剂量托烷司琼对顺铂引起的大鼠异嗜高岭土行为的影响。方法96只健康成年SPF级雄性Wistar大鼠按随机数字表法分为8组（n=12）：Tc、T10、T20、T30、Ic、I0.3、I0.5和I0.7组，其中TC、T10、T20、T30组分别于鞘内给与生理盐水30μl和托烷司琼10、20、30μg；Ic、I0.3、I0.5和I0.7组分别于静脉给生理盐水0．5ml和托烷司琼0．3、0．5和0．7mg／kg，30min后腹腔注射顺铂3mg／kg，建立顺铂异嗜高岭土行为动物模型，观察顺铂给药后48h高岭土饲料和正常饲料摄取量以及大鼠体重变化。结果与TC组比较，k组高岭土摄入量明显减少、食物摄取量显著增加、体重下降程度明显减少（P〈0．05）；与Ic组比较，I0.5和I0.7组高岭土摄取量明显减少、食物摄取量显著增加、体重减轻程度明显减少（P〈0．05），但I0.7组与I0.5组组间比较差异无统计学意义（P〉0．05）；T如组与I0.5和I0.7组组间比较各指标差异无统计学意义（P〉0．05）。结论鞘内给予托烷司琼20雌能显著减少大鼠高岭土摄取量，降低其体重减轻程度，并能使大鼠进食量增加，且与静脉给予0．5ms／kg和0．7mg／kg疗效相当，鞘内给予托烷司琼可以作为给药途径之一。

Objective To compare the effects of different administration routes and dosages of tropisetron on cisplatin-induced kaolin intake in rats. Methods Ninety - six healthy adult male Wistar SPF rats were randomly divided into 8 groups（ n = 12 each） ： intrathecal （IT） control group （ group Tc ） and 3 tropisetron groups receiving IT tropisetron 10, 20 and 30μg, the volume of each group was 30 μl （group T10, T20, T30 ）, intravenous（IV） control group （group Ic ） and 3 tropisetron groups receiving IV tropisetron 0. 3, 0. 5 and 0. 7 mg/kg respectively （group I0.3, I0.5, I0.7 ）. In group Tc and Ic, normal saline 30 ~1 and 0. 5 ml were injected IT and IV, respectively. All rats received cisplatin 3mg/kg by intraperitoneal injection at the time point of thirty minutes after administration, each rat weight, the daily food and kaolin intakes were detected at the time point of 48 hours after cisplatin administration. Results Compared with group Tc, each rat weight loss, the kaolin intakes were significantly decreased （ P 〈 0. 05 ）, and food intake dose was significantly increased in group T20 （ P 〈 0. 05）. Compared with group Ic, each rat weight loss, the kaolin intakes were significantly decreased （ P 〈 0.05）, and food intake dose was significantly increased in group I0. 5 and I0.7 （ P 〈 0. 05）. There was no significant difference between group I0.5, I0. 7 and group T20. Conclusions The kaolin intakes and the rat weight loss can be decreased by IT tropisetron, and the food take dose was increased meanwhile, and IT tropisetron 20 μg has equivalent efficacy to IV tropisetron 0. 5 or 0. 7 mg/kg. IT could be the new administration route of tropisetron.