人骨髓间充质干细胞培养上清与肝星状细胞相关酶的分泌

Effect of human bone marrow mesenchymal stem cells culture supernatant on related enzyme expression in hepatic stellate cells

背景:研究证实骨髓间充质干细胞移植治疗能改善甚至逆转肝纤维化,但其具体机制尚不清楚。目的:检测人骨髓间充质干细胞培养上清对肝星状细胞中基质金属蛋白酶2及组织抑制基质金属蛋白酶1表达的影响。方法:采用密度梯度离心法分离人骨髓间充质干细胞,收集原代培养7d的骨髓间充质干细胞培养上清,加入肝星状细胞中培养作为实验组,并设置单独肝星状细胞组、单独骨髓间充质干细胞组为对照。培养24,48h后,检测各组细胞上清中基质金属蛋白酶2蛋白与组织抑制基质金属蛋白酶1蛋白的表达。结果与结论:与单独肝星状细胞组、单独骨髓间充质干细胞组比较,实验组培养24,48h后肝星状细胞基质金属蛋白酶2及组织抑制基质金属蛋白酶1表达减少(P<0.05或P<0.01)。表明骨髓间充质干细胞培养上清抑制肝星状细胞中基质金属蛋白酶2、组织抑制基质金属蛋白酶1的表达。

BACKGROUND： The studies have proved that bone marrow mesenchymal stem cells transplantation therapy can improve or even reverse the liver fibrosis, but its mechanism is still unclear. OBJECTIVE： To detect the effect of human bone marrow mesenchymal stem cells culture supernatant on the expression of matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 1 in hepatic stellate cells. METHODS： The density gradient centrifugation was used to isolate human bone marrow mesenchymal stem cells and collected the culture supernatant of human bone marrow mesenchymal stem cells after cultured for 7 days, the human bone marrow mesenchymal stem cells culture supematant added with the hepatic stellate cells was considered as experimental group, a simple hepatic stellate cells group and simple human bone marrow mesenchymal stem cells group were set up as control groups. The expressions of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the culture supernatant of three groups were detected after cultured for 24 and 48 hours. RESULTS AND CONCLUSION： The expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the experimental group was decreased when compared with simple hepatic stellate cells group and simple human bone marnow mesenchymal stem cells group （P 〈 0.05 or P 〈 0.01）. The results showed that bone marrow mesenchymal stem cells culture supernatant can inhibit the expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in hepatic stellate cells.