股骨近端Stro-1^+细胞与骨形态发生蛋白及其受体的相关性

Correlation between Stro-1^+ cells and bone morphogenetic protein and its receptor in proximal femur

背景:髋关节置换后假体的融合率与假体周围的骨重建密切相关,这个过程中骨髓间充质干细胞的募集、向成骨细胞分化的途径受骨形态发生蛋白调控。目的:分析股骨近端Stro-1+细胞与骨形态发生蛋白7、骨形态发生蛋白受体的相关性。方法:32例患者初次进行髋关节置换,取术中股骨矩开槽时骨块,体外细胞培养14d后检测骨髓间充质干细胞数量,Stro-1+标记干细胞的总量,骨形态发生蛋白7、骨形态发生蛋白受体1a、骨形态发生蛋白受体2的表达。结果与结论:Stro-1+、骨形态发生蛋白7、骨形态发生蛋白受体1a、骨形态发生蛋白受体2的表达在不同年龄组有轻微差异,但差异无显著性意义,在不同性别组差异也无显著性意义;股骨近端Stro-1+细胞数量(即代表具有向成骨细胞分化功能的细胞)与骨形态发生蛋白7表达呈负相关关系,与骨形态发生蛋白受体1a表达呈正相关关系,其可以影响髋关节假体的骨融合与生存率。

BACKGROUND： A successful osseointegration after total hip arthroplasty relies on the bone reconstruction around the prosthesis. Bone marrow mesenchymal stem cells are recruited and the commitment of bone marrow mesenchymal stem cells to an osteoblastic differentiation pathway is apparently under the control of bone morphogenic proteins. OBJECTIVE： To analyze the correlation between Stro-1^＋ bone marrow mesenchymal stem cells and bone morphogenetic protein 7 and bone morphogenetic protein receptor in proximal femur. METHODS： Bone samples were obtained from the discarded metaphysis region of the proximal femur in 32 patients with primary total hip arthroplasty, and the number of bone marrow mesenchymal stem cells was measured after in vitro cultured for 14 days. Stro-1^＋ was used to mark the stem cells. The expression of bone morphogenetic protein 7, bone morphogenetic protein receptor la and bone morphogenetic protein receptor 2 was detected. RESULTS AND CONCLUSION： There were differences of Stro-1^＋ cells, bone morphogenetic protein 7, bone morphogenetic protein receptor la and bone morphogenetic protein receptor 2 expression between different years, but the differences were not significant, and there was no significant difference between genders. There was a negative correlation between the numbers of Stro-1^＋ cells in proximal femur and expression of bone morphogenetic protein 7 that means the cell had the function to differentiate into osteoblasts. The correlation between the number of Stro-1^＋ cells and expression of bone morphogenetic protein receptor la was positive which can influence the fusion rate and the survival rate of the hip prosthesis.