骨髓间充质干细胞治疗早期骨性关节炎软骨T2值及相关因子的表达

Changes in T2 value and related factor expression after treatment of early osteoarthritis by bone marrow mesenchymal stem cells transplantation

背景:血清肿瘤坏死因子α和白细胞介素1β作为炎症递质,其水平与骨性关节炎病情相关。而骨髓间充质干细胞治疗骨性关节炎报道较少。目的:用骨髓间充质干细胞治疗兔早期骨性关节炎,观察关节软骨T2值及血清肿瘤坏死因子α和白细胞介素1β水平并进行分析。方法:将36只新西兰大白兔随机均分成对照组、模型组和治疗组。模型组兔仅建立骨性关节炎模型,治疗组在造模后第4周左膝关节腔内注射骨髓间充质干细胞;对照组膝关节腔内注射等量生理盐水。分别在治疗后第2周,1,2,3个月行左后膝关节核磁共振及血清肿瘤坏死因子α和白细胞介素1β水平检查。结果与结论:与模型组比较,治疗组T2值降低,无关节腔积液;血清肿瘤坏死因子α和白细胞介素1β水平降低;软骨T2值变化与二者呈正相关。MRI能够反映早期关节软骨的生物学改变,可用于评价骨性关节炎治疗前后的改变;骨髓间充质干细胞在关节腔内可向软骨分化,为早期骨性关节炎患者提供了一种新的有效疗法。

BACKGROUND： Serum tumor necrosis factor α and interleukin-1β as an inflammatory mediator is related to the condition of osteoarthritis. However, the reports on the treatment of osteoarthritis by bone marrow mesenchymal stem cells are rare. OBJECTIVE： To observe the T2 value, the serum tumor necrosis factor α and interleukin-1β levels in the treatment of early osteoarthritis in rabbits by bone marrow mesenchymal stem cells transplantation. METHODS： Thirty-six New Zealand white rabbits were randomly and equally divided into control group, model group and treatment group. Rabbits in the model group were only used to establish the osteoarthritis model; left knee joint cavity of rabbits in the treatment group was injected with bone marrow mesenchymal stem cells at 4 weeks after modeling; knee joint cavity of rabbits in the control group was injected with normal saline in the same dose. Left knee joint magnetic resonance imaging and the detection of serum tumor necrosis factor α and interleukin-1β levels were performed at 2 weeks, 1, 2 and 3 months after treatment. RESULTS AND CONCLUSION： Compared with the the serum tumor necrosis factor α and interleukin-1β meodel group, the T2 value reduced, without arthroedema, and levels were decreased in the treatment group. The T2 value was positive correlated with serum tumor necrosis factor α and interleukin-1β levels. MRI can reflect the early biologically change of articular cartilage, and it can be used to evaluate the curative effect in the early stages of osteoarthritis. Bone marrow mesenchymal stem cells can differentiate into chondrocytes in articular cavity and is effective for early osteoarthritis in rabbits.