脐带来源间充质干细胞在成脂培养条件下骨形态发生蛋白2的表达

Bone morphogenetic protein 2 expression in umbilical cord mesenchymal stem cells after adipogenic induction

背景:脐带间充质干细胞在成脂培养条件下成脂相关信号因子过氧化物酶体增殖体激活受体γ的表达水平增高,同时成骨相关信号因子骨形态发生蛋白的表达水平也增高。目的:检测成脂诱导条件下脐带间充质干细胞的过氧化物酶体增殖体激活受体γ及骨形态发生蛋白2的表达水平变化,并对结果进行初步探讨。方法:取脐带来源的间充质干细胞,以成脂诱导体系对细胞进行诱导分化培养,倒置显微镜下观察细胞体外培养生长情况;油红O染色法观察成脂现象,茜素红及vonkossa染色观察是否有沉淀形成;荧光定量PCR方法检测成脂相关因子过氧化物酶体增殖体激活受体γ及骨形态发生蛋白2的表达变化情况。结果与结论:获得脐带来源间充质干细胞,并成功进行了成脂诱导分化。荧光定量PCR检测发现在成脂诱导条件下脐带间充质干细胞的过氧化物酶体增殖体激活受体γ和骨形态发生蛋白2的表达水平全都呈升高趋势,但是前者对分化方向的调控起主要作用。

BACKGROUND： Expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptor Y gene in umbilical cord mesenchymal stem cells after adipogenic induction is increased at the same time. OBEJECTIVE： To investigate the changes of bone morphogenetic protein 2 and peroxisome proliferator-activated receptor Y gene expression in umbilical cord mesenchymal stem cells after adipogenic induction, and to compare the results METHODS： With specific inductive medium, umbilical cord mesenchymal stem cells were obtained and then induced into adipogenisis. Their morphological characteristics were observed with inverted microscope. Oil red O staining was used to observe the adipogenic phenomenon, and von kossa staining and alizarin red staining were used to identify the formation of precipitation. Real-time polymerase chain reaction was used to quantify the gene expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptor Y at various time points after adipogenic induction. RESULTS AND CONCLUSION： Umbilical cord mesenchymal stem cells could be isolated and cultured successfully. After cultured in adipogenic medium, the cells differentiated into adipogenisis. The result of real-time polymerase chain reaction showed that both the expression levels of bone morphogenetic protein 2 and peroxisome proliferator-activated receptor Y gene were increased after adipogenic induction, but peroxisome proliferator-activated receptor Y played a major role in the regulation of differentiation.