非黏附骨髓间充质干细胞移植改善脑缺血小鼠的学习记忆功能

Transplantation of non-adherent mesenchymal stem cells improves the learning and memory functions of mice with cerebral ischemia

背景:非黏附骨髓间充质干细胞在体外培养中具有自我更新能力,可诱导分化为脂肪细胞、成骨细胞和软骨细胞,表现出多分化潜能,在移植到缺血损伤脑内可存活并且部分可分化为神经细胞或神经胶质细胞。目的:观察小鼠非黏附骨髓间充质干细胞移植到缺血小鼠脑内对神经功能的修复作用。方法:全骨髓法分离β-Gal转基因小鼠总骨髓细胞,采用反复转移非黏附骨髓细胞的培养法收集纯化的第3代非黏附骨髓间充质干细胞。20只C57BL/6J小鼠建立大脑中动脉阻塞模型,随机区组法分为2组,造模后7d,细胞移植组将第3代非黏附骨髓间充质干细胞悬液3μL定向移植到小鼠脑损伤处,对照组注射等量生理盐水。移植28d后进行Morris水迷宫实验,连续8d。结果与结论:在定位航行实验中细胞移植组小鼠的逃避潜伏期及游泳总路程均较对照组明显缩短(P<0.05),空间探索实验中细胞移植组小鼠穿越原平台次数较对照组明显增多(P<0.05)。提示非黏附骨髓干细胞移植能够提高脑缺血模型小鼠的学习记忆能力,改善神经功能。

BACKGROUND： Non-adherent mesenchymal stem cells exhibit the capacity of self-renewal and can be induce-differentiated into adipocytes, osteoblasts and chondrocytes. Non-adherent mesenchymal stem cells can survive and partial non-adherent mesenchymal stem cells can differentiate into neural cells or glial cells after transplantation into ischemic brain regions. OBJECTIVE： To investigate the effect of non-adherent mesenchymal stem cells transplanted into ischemic brain regions on recovery of neurological function. METHODS： Total bone marrow cells from β-Gal transgenic mice were separated by the whole bone marrow method. Passage 3 non-adherent mesenchymal stem cells were collected and purified by repeated transfer method. Twenty C57BL/6L mice were established into models of occlusion of middle cerebral artery and then randomized into two groups In the cell transplantation group, 3μL passage 3 non-adherent mesenchymal stem cells suspension was injected into the ischemic brain region at 7 days after middle cerebral artery occlusion. At the same time, equal amount of physiological saline was injected into the control group. Since 28 days after cell transplantation, the learning and memory functions of mice were determined by 8-day Morris water maze test. RESULTS AND CONCLUSION： In the place navigation test, the escape latency and swim length of mice in the cell transplantation group were significantly shortened than those in the control group （P 〈 0.05）. In the probe trial, the number that mice passed cross the platform in the cell transplantation group was significantly greater than that in the control group （P 〈 0.05）. Transplantation of non-adherent mesenchymal stem cells can improve the learning and memory functions of mice with cerebral ischemia.