苗药九仙罗汉接骨汤促进胫骨干骨折的愈合

Miao Nationality's Jiuxianluohanjiegu decoction promotes tibial shaft fracture healing

背景:临床研究表明苗药九仙罗汉接骨汤具有活血散瘀通络,接骨消肿止痛之功效,但其促进骨折愈合的作用机制尚未见相关报道。目的:观察苗药九仙罗汉接骨汤促进兔胫骨干骨折愈合的效果。方法:新西兰大白兔右胫骨前部中断造成宽3mm深2mm的骨缺损模型,随机分为3组:接骨汤组,三七对照组,生理盐水组,分别灌胃苗药九仙罗汉接骨汤煎剂、三七粉液、生理盐水。所有动物于骨折当天开始给药,于给药1,7,14,21,28d时测定全血比黏度、血浆比黏度、红细胞压积、血钙、血磷、碱性磷酸酶水平,X射线评分及病理学变化。结果与结论:接骨汤组与三七对照组、接骨汤组与生理盐水组两两相比较,血清钙、血清磷、血清碱性磷酸酶水平明显升高并提前出现峰值,以后逐渐下降(P<0.05),血液流变学各项指标水平逐渐降低(P<0.05)。X射线片评分半定量分析显示,九仙罗汉接骨汤组骨折愈合速度明显快于三七对照组和生理盐水组,差异有显著性意义(P<0.05)。苏木精-伊红染色显示九仙罗汉接骨汤组的骨痂形成速度和质量明显高于其他两组。结果可见苗药九仙罗汉接骨汤具有促进其骨痂生成,缩短骨折愈合时间的作用。

BACKGROUND：Clinical researches have shown that Miao Nationality＇s Jiuxianluohanjiegu decoction has the effect of promoting the blood circulation,remove blood stasis and obstruction,and reunion of bone,detumescence and analgesia,but the report on the effect in promoting the fracture healing is rare.OBJECTIVE：To observe the effect of Miao Nationality＇s Jiuxianluohanjiegu decoction in promoting the rabbit tibial shaft fracture healing.METHODS：Using traditional Chinese medicine method of decocting,preparing experiment required concentration of decoction,millipore filtration standby.New Zealand rabbit white right tibialis anterior disruption caused by width 3 mm and depth 2 mm of the bone defect model,and the models were randomly divided into three groups;Jiegu decoction group,Sanqi control group,normal saline group.The rabbits in the three groups were gavaged with Miao Nationality＇s Jiuxianluohanjiegu decoction,Sanqi powder and liquid and normal saline respectively.All the rabbits were administrated since fracture,and the whole blood viscosity,plasma viscosity,red blood cells deposited,blood calcium,serum phosphorus,alkaline phosphatase concentration,X-ray score and pathological changes were measured at 1,7,14,21 and 28 days after administration.RESULTS AND CONCLUSION：Compared with Sanqi control group and normal saline group,the blood calcium,serum phosphorus and alkaline phosphatase concentration in the Jiegu decoction group were increased and reached to peak early,then gradually decreased（P0.05）,while the hemorheology indicators were gradually decreased（P0.05）.X-ray score half quantitative analysis showed that the fracture healing of the Jiegu decoction group was faster than that of the Sanqi control group and normal saline group,and the difference was significant（P0.05）.Hematoxylin-eosin staining revealed that the callus formation speed and quality in the Jiegu decoction group were much higher than those in the other two groups.Miao Nationality＇s Jiuxianluohanjiegu decoction can promote the callus formation and shorten the fracture healing time.