张力对小鼠增生性瘢痕形成及羟脯氨酸含量的影响

Effect of mechanical strain on hypertrophic scar formation and hydroxyproline content in mice

背景:张力在细胞增殖、分化、凋亡以及基因表达过程中起着重要的作用。目的:观察张力对小鼠增生性瘢痕形成及羟脯氨酸含量的影响。方法:4周龄大C57/BL小鼠随机分为2组,张力组小鼠背部制作一个2cm长直线性全层皮肤切口,用尼龙线缝合,4d后拆线,再用尼龙线将22-mm扩张螺丝固定到已经愈合的创面上,隔日扩张。对照组扩张螺丝不动。分别于伤后1,2,3,4,5周各取6只小鼠瘢痕组织观察瘢痕组织厚度、横截面积改变,测量瘢痕组织羟脯氨酸含量。结果与结论:张力组瘢痕增生明显,其厚度、横截面积显著大于对照组(P<0.01),张力组各时间点瘢痕组织羟脯氨酸含量显著高于对照组(P<0.01)。表明张力导致小鼠瘢痕增生显著,羟脯氨酸含量显著增高。

BACKGROUND：Mechanical strain plays a key role in cell proliferation,differentiation,apoptosis and gene expression.OBJECTIVE：To investigate mechanical strain effects on the formation of hypertrophic scar and hydroxyproline content in mice.METHODS：Four weeks old C57/BL mice were randomly divided into mechanical strain group and control group.The mice in the mechanical strain group were subjected to a 2-cm full-thickness skin incision,and then sutured with nylon wire.After stitches were taken out at day 4,222-cm-mm stretching screw was fixed on the healed wound with nylon wire and expanded every other day.While stretching screw in the control group had no treatment.Six mice in each group were collected respectively at weeks 1,2,3,4 and 5 after wounded,and then their hypertrophic scar thickness,cross-sectional area changes and hydroxyproline content in the hypertrophic scar tissues were observed.RESULTS AND CONCLUSION：Compared with the control group,scar hyperplasia in the mechanical strain group was more obvious,besides,the thickness,cross-sectional area of hypertrophic scars was bigger（P0.01）.Hydroxyproline content in the hypertrophic scar tissues of the mechanical strain group was significantly higher than that in the control group at each time point（P0.01）.These results suggest that mechanical strain can result in obvious scar hyperplasia in mice and a significant increase in hydroxyproline content.