摘要
背景:实验发现链球菌溶血素O引起的细胞通透在一定程度上是可逆的,链球菌溶血素O产生的孔道在Ca2+存在的情况下可以自然封闭。目的:观察链球菌溶血素O是否可以使猪成纤维细胞的通透性发生改变,及不同质量浓度的链球菌溶血素O对细胞状态的影响。方法:分别使用质量浓度为0,230,300,400μg/L的链球菌溶血素O溶液处理猪成纤维细胞,孵育50min后,加入PI染液,观察细胞的通透率。加入含Ca2+的培养液封闭细胞后,分别观察细胞的形态变化及增殖能力的变化。结果与结论:随着链球菌溶血素O溶液质量浓度的增加,细胞的通透率只轻度提高。链球菌溶血素O处理细胞后,细胞的形态没有发生明显的改变。质量浓度为230μg/L的链球菌溶血素O溶液对细胞的增殖能力无明显影响,而300μg/L和400μg/L的链球菌溶血素O溶液明显地降低了细胞的增殖能力。提示质量浓度为230μg/L的链球菌溶血素O溶液可以提高细胞的通透性,并保持细胞正常的形态及增殖能力。
BACKGROUND:Streptolysin O(SLO)-induced cell permeability is reversible to a certain extent,and the pores can be closed in the medium containing Ca2+.OBJECTIVE:To observe whether SLO could improve the permeability of pig fibroblasts and to observe the states of cells treated with SLO of different concentrations.METHODS:Pig fibroblasts were permeated with SLO solution of different concentrations(0,230,300 and 400μg/L respectively).After incubation of 50 minutes,the cells were stained with PI to observe cell permeability.When adding the medium containing Ca2+,the cells were plated.Morphology and multiplication capacity of these cells were detected.RESULTS AND CONCLUSION:Along with increase of SLO solution concentration,the cell permeability improved slightly.When cells were treated with SLO,the cell morphology was not changed.230μg/L SLO had no influence on the capacity of cell proliferation,and however,300μg/L and 400μg/L SLO reduced the proliferation capacity significantly appearing statistically difference when compared with 0μg/L group.These findings indicate that 230μg/L SLO could increase cell permeability with keeping the normal shape and proliferation ability of cells.
出处
《中国组织工程研究》
CAS
CSCD
2012年第46期8689-8692,共4页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(30900308)~~