链球菌溶血素O提高细胞的通透性

Effects of streptolysin O on increasing cell permeability

背景:实验发现链球菌溶血素O引起的细胞通透在一定程度上是可逆的,链球菌溶血素O产生的孔道在Ca2+存在的情况下可以自然封闭。目的:观察链球菌溶血素O是否可以使猪成纤维细胞的通透性发生改变,及不同质量浓度的链球菌溶血素O对细胞状态的影响。方法:分别使用质量浓度为0,230,300,400μg/L的链球菌溶血素O溶液处理猪成纤维细胞,孵育50min后,加入PI染液,观察细胞的通透率。加入含Ca2+的培养液封闭细胞后,分别观察细胞的形态变化及增殖能力的变化。结果与结论:随着链球菌溶血素O溶液质量浓度的增加,细胞的通透率只轻度提高。链球菌溶血素O处理细胞后,细胞的形态没有发生明显的改变。质量浓度为230μg/L的链球菌溶血素O溶液对细胞的增殖能力无明显影响,而300μg/L和400μg/L的链球菌溶血素O溶液明显地降低了细胞的增殖能力。提示质量浓度为230μg/L的链球菌溶血素O溶液可以提高细胞的通透性,并保持细胞正常的形态及增殖能力。

BACKGROUND：Streptolysin O（SLO）-induced cell permeability is reversible to a certain extent,and the pores can be closed in the medium containing Ca2＋.OBJECTIVE：To observe whether SLO could improve the permeability of pig fibroblasts and to observe the states of cells treated with SLO of different concentrations.METHODS：Pig fibroblasts were permeated with SLO solution of different concentrations（0,230,300 and 400μg/L respectively）.After incubation of 50 minutes,the cells were stained with PI to observe cell permeability.When adding the medium containing Ca2＋,the cells were plated.Morphology and multiplication capacity of these cells were detected.RESULTS AND CONCLUSION：Along with increase of SLO solution concentration,the cell permeability improved slightly.When cells were treated with SLO,the cell morphology was not changed.230μg/L SLO had no influence on the capacity of cell proliferation,and however,300μg/L and 400μg/L SLO reduced the proliferation capacity significantly appearing statistically difference when compared with 0μg/L group.These findings indicate that 230μg/L SLO could increase cell permeability with keeping the normal shape and proliferation ability of cells.