槲皮素壳聚糖溶液的抗炎镇痛作用

Anti-inflammatory and analgesic effects of quercetin chitosan composite solution

背景:前期实验发现槲皮素壳聚糖膜对口腔溃疡有明显治疗作用。目的:验证槲皮素壳聚糖溶液的抗炎镇痛作用。方法:设计3组实验,均将SD大鼠或昆明小鼠随机分为模型组、阳性对照组、壳聚糖对照组与实验组。①角叉菜胶致小鼠足趾肿胀实验:将各组大鼠双足分别浸泡在生理盐水、冰硼散溶液、壳聚糖溶液、槲皮素壳聚糖溶液中,再进行角叉菜胶足部注射。②巴豆油致小鼠耳郭肿胀实验:在各组大鼠耳部分别涂抹上述药物,再进行巴豆油耳部涂抹。③热板法致疼痛实验:在各组大鼠足底分别涂抹上述药物,涂抹前后行热板刺激实验。结果与结论:与模型组比较,实验组可明显抑制大鼠足部、耳部肿胀及足部疼痛(P<0.05,P<0.01),提高痛阈值和镇痛百分率(P<0.05,P<0.01),且效果优于阳性对照组与壳聚糖对照组。证明槲皮素壳聚糖溶液具有明显的抗炎镇痛作用。

BACKGROUND： Early experiments have shown that quercetin chitosan membrane has obvious therapeutic effect on oral ulcer OBJECTIVE： To verify the anti-inflammatory and analgesic effects of quercetin chitosan composite solution METHODS： SD rats or Kunming mice were randomly divided into four groups： control group, chitosan group, quercetin chitosan composite solution group and Bingpeng San group. ①Experiment of rat foot swelling induced by carrageenin The feet in each group of rats were immersed in normal saline, chitosan solution, quercetin chitosan solution and Bingpeng San solution. And then the rats were administered with carrageenin foot injection.②Experiment of rat ear swelling induced by croton oil： The ears of rats in each group were treated with the above drugs, and then given the croton oil in ears. ③Experiment of rat pain induced by hot plate： Foot sole of rats in each group was smeared with the above drugs and given hot plate stimulation experiments before and after application. RESULTS AND CONCLUSION： Compared with the control group, the quercetin chitosan composite solution group significantly inhibited the ear and foot swelling of rats as well as foot pain （P 〈 0.05, P 〈 0.01 ）.Besides, pain threshold and analgesic pain killer was increased （P 〈 0.05, P 〈 0.01 ）. What＇s more, the effect in the quercetin chitosan composite solution group was better than that in the Bingpeng San group and chitosan group. These results suggest that quercetin chitosan composite solution has obvious anti-inflammatory and analgesic effects.